CAJ | 학술논문

目的观察真武汤冲剂对大鼠慢性压力负荷性心肌肥厚的影响.方法租用广州呼吸疾病研究所实验室,将SD大鼠随机(随机数字法)分为3组,假手术组8只,手术组和中药组各15只.手术组和中药组行腹主动脉缩窄术,制作心肌肥厚模型.16周行心脏超声,确定术后心肌肥厚,中药组加用真武汤混合饲料喂养,24周时用高频超声测定各组心脏质量、结构和功能,以及心肌组织Mas-son染色,测定总胶原容积百分比(CVF-T)和无冠状动脉小血管视野胶原容积百分比(CVF-NV).各组数据采用SNK法进行两两比较.结果手术组心脏质最增加,以左室增加为明显(P＜0.05),心脏增大,以舒张期室间隔(IVSd)和左室后壁(LVPWd)为明显(P＜0.01),心功能明显降低(P＜0.05);中药组相对于手术组,心脏质量减轻(P＜0.01),体积缩小,以IVSd和收缩期左室后壁(LVPWs)为明显(P＜0.01),心功能改善(P＜0.05).病理分析手术组间质和胶原增生明显,中药组有不同程度改善,CVF-T和CVF-NV较手术组组明显减少(P＜0.01),与假手术组相比,差异尤统计学意义(P＞0.05).结论真武汤冲剂能够减少压力负荷性大鼠的心脏质量、体积,改善心功能,抑制胶原纤维增生,逆转心肌肥厚.
목적 관찰진무탕충제대대서만성압력부하성심기비후적영향.방법 조용엄주호흡질병연구소실험실,장SD대서수궤(수궤수자법)분위3조,가수술조8지,수술조화중약조각15지.수술조화중약조행복주동맥축착술,제작심기비후모형.16주행심장초성,학정술후심기비후,중약조가용진무탕혼합사료위양,24주시용고빈초성측정각조심장질량、결구화공능,이급심기조직Mas-son염색,측정총효원용적백분비(CVF-T)화무관상동맥소혈관시야효원용적백분비(CVF-NV).각조수거채용SNK법진행량량비교.결과 수술조심장질최증가,이좌실증가위명현(P＜0.05),심장증대,이서장기실간격(IVSd)화좌실후벽(LVPWd)위명현(P＜0.01),심공능명현강저(P＜0.05);중약조상대우수술조,심장질량감경(P＜0.01),체적축소,이IVSd화수축기좌실후벽(LVPWs)위명현(P＜0.01),심공능개선(P＜0.05).병리분석수술조간질화효원증생명현,중약조유불동정도개선,CVF-T화CVF-NV교수술조조명현감소(P＜0.01),여가수술조상비,차이우통계학의의(P＞0.05).결론 진무탕충제능구감소압력부하성대서적심장질량、체적,개선심공능,억제효원섬유증생,역전심기비후.
Objective To investigate the effects of Zhenwu decoction (a herbal preparation made in a form of instant granules readily soluble into decoction to administer) on rats with chronic pressure-overloaded left ven-tricular hypertrophy. Method All the male SD rats were randomly(random number) divided into 3 groups: sham operation group (n= 8), operation group (n= 15) and herbal medicine group (n= 15). The model of myocar-dial hypertrophy was made by gradually constricting the abdominal aorta. After 16 weeks, cardiac ultrasonography was used in rats of all groups to ascertain the post-operational left ventricular (LV) hypertrophy established. And the Zhenwu decoction was added in the herbal medicine group in a dose of 12 g/kg mixed in feedstuff fed for 8 weeks. After 24 weeks, the weight and structure as well as function of the heart of rats in each group were mea-sured by high-frequency ultrasonography, and Massion stain was performed on cardiac muscles. Meanwhile, both the total collagen volume fraction (CVF-T) and non-coronary vessel collagen volume fraction (CVF-NV) were ana-lyzed. Results There was an increase in the weight of the heart with a predominance of the left ventricle in the operation group (P ＜ 0.05) . The heart was enlarged with diastolic interventricular septum thickness (IVSt) and left ventricular posterior wall thickness (LVPWt) increased predominantly (P ＜ 0.01). There was a significant decrease in cardiac function (P ＜ 0.05). In herbal medicine group, both the weight and volume of heart were lower than those in operation group with predominance of IVSt and systolic left ventricular wall (P ＜ 0.01 both).And the cardiac function of rats in herbal group was better (P ＜ 0.05). Interstitial and collagen hyperplasia increased significantly in the rats of operation group found by pathological analysis. In herbal medicine group, the pathological changes were blunted in varied magnitude with significant decrease in CVF-T and CVF-NV in compari-son with operation group (P＜0.01), and no difference in those was found compared with sham operation group (P＞ 0.05) . Conclusions The Zhenwu decoction could minimize the hyperplasia changes of heart in rats with pressure-overloaded left ventricular hypertrophy, maintaining the normal cardiac function.