CAJ | 학술논문

目的采用HAIN基因分型试剂盒鉴定临床非结核分枝杆菌,评价其优缺点.方法收集浙江、安徽等地74株临床非结核分枝杆菌,采用HAIN基因分型试剂盒鉴定临床非结核分枝杆菌,并用16S rRNA基因测序方法对其进行比较和评价.结果 74株非结核分枝杆菌HAIN基因分型试剂盒鉴定结果为:31株胞内分枝杆菌,12株脓肿分枝杆菌,8株偶发分枝杆菌,6株堪萨斯分枝杆菌,5株鸟分枝杆菌,3株耻垢分枝杆菌,2株草分枝杆菌,2株瘰疬分枝杆菌,1株戈登分枝杆菌,另外有4株菌株只能鉴定为分枝杆菌属,不能鉴定到种.8株结核分枝杆菌鉴定准确.与16SrRNA基因测序相比较,HAIN基因分型试剂盒除了4株菌株只能鉴定为分枝杆菌属以外,其余70株非结核分枝杆菌均能鉴定到种,鉴定符合率为94.59％；并且它能进一步区分脓肿分枝杆菌和龟分枝杆菌,以及堪萨斯分枝杆菌和胃分枝杆菌.如果单纯鉴定HAIN基因分型试剂盒菌株范围之内的临床最常见非结核分枝杆菌菌株,鉴定符合率为100％.结论 HAIN基因分型试剂盒鉴定临床非结核分枝杆菌时间短、操作简单、结果准确,可在临床推广使用.
목적 채용HAIN기인분형시제합감정림상비결핵분지간균,평개기우결점.방법 수집절강、안휘등지74주림상비결핵분지간균,채용HAIN기인분형시제합감정림상비결핵분지간균,병용16S rRNA기인측서방법대기진행비교화평개.결과 74주비결핵분지간균HAIN기인분형시제합감정결과위:31주포내분지간균,12주농종분지간균,8주우발분지간균,6주감살사분지간균,5주조분지간균,3주치구분지간균,2주초분지간균,2주라력분지간균,1주과등분지간균,령외유4주균주지능감정위분지간균속,불능감정도충.8주결핵분지간균감정준학.여16SrRNA기인측서상비교,HAIN기인분형시제합제료4주균주지능감정위분지간균속이외,기여70주비결핵분지간균균능감정도충,감정부합솔위94.59％；병차타능진일보구분농종분지간균화구분지간균,이급감살사분지간균화위분지간균.여과단순감정HAIN기인분형시제합균주범위지내적림상최상견비결핵분지간균균주,감정부합솔위100％.결론 HAIN기인분형시제합감정림상비결핵분지간균시간단、조작간단、결과준학,가재림상추엄사용.
Objective To identify non-tuberculous Mycobacterium(NTM) rapidly with HAIN molecular assay genotype Mycobacterium kit,and investigate the advantages and disadvantages of this method.Methods Seventy-four clinical NTM isolates were collected from hospitals in Zhejiang and Anhui province.Clinical strains were identified with HAIN molecular assay genotype Mycobacterium kit.16S rRNA gene sequencing was used to estimate and compare with this method.Results The results of kit showed that there were thirty-one M.intracellulare strains,twelve M.chelonae strains,eight M.fortuitum strains,six M.kansasii strains,five M.avium strains,three M.smegmatis strains,two M.phlei strains,two M.scrofulaceum strains and one M.gordon strain.Four strains were identified as Mycobacterium without further identification.Eight M.tuberculosis strains were identified correctly too.Compared with 16S rRNA gene sequencing,except for four strains identified as Mycobacterium,others 70 strains got the same results as 16S rRNA gene sequencing,the coincidence was 94.59％,and it could further identify thirteen Mycobacterium chelonae complex and eight Mycobacterium kansasii complex to subspecies M.abscessus and M.kansasii,respectively.If only to identify strains under the identification range of this kit,the coincidence reach to 100％,Conclusion The method of HAIN molecular assay genotype Mycobacterium kit is simple and accurate,the time is shorter and should widely be applied clinically.