中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2011年
10期
889-892
,共4页
井娜%吕滨%康金锁%张春玲%鲁锦国%张丽%陈雄彪%侯志辉%陈曦%丛祥凤
井娜%呂濱%康金鎖%張春玲%魯錦國%張麗%陳雄彪%侯誌輝%陳晞%叢祥鳳
정나%려빈%강금쇄%장춘령%로금국%장려%진웅표%후지휘%진희%총상봉
冠状动脉疾病%基质金属蛋白酶9%过氧化物酶%CD40配体%体层摄影术,螺旋计算机
冠狀動脈疾病%基質金屬蛋白酶9%過氧化物酶%CD40配體%體層攝影術,螺鏇計算機
관상동맥질병%기질금속단백매9%과양화물매%CD40배체%체층섭영술,라선계산궤
Coronary artery disease%Matrix metalloproteinase 9%Peroxidase%CD40 ligand%Tomography,spiral computed
目的 探讨MMP9、MPO及sCD40L在识别冠状动脉斑块性质中的作用.方法 选取2008年4月至2010年1月阜外心血管病医院门诊胸痛患者118例,根据64排螺旋CT检查结果,将CT值＜130 Hu的患者入选为非钙化斑块组(71例),CT值≥130 Hu的患者入选为钙化斑块组(47例).选取90名健康体检者为对照组.采用ELISA检测血清MMP9、MPO及sCD40L水平,并比较其在各组中水平的差异.采用ROC曲线评价各标志物诊断非钙化斑块的敏感度和特异度.结果 非钙化斑块组血清MMP9、MPO、sCD40L水平分别为(762.25±368.71)、[844.10(582.00～ 1220.70)]、(9.37±3.15) μg/L,高于健康对照组的(342.70±178.53)、[426.35(283.20～592.00)]、(6.55±2.96) μg/L及钙化斑块组的(483.12±219.09)、[469.00(302.45～723.55)]、(7.24±2.86) μg/L,差异均有统计学意义(统计值分别为F =42.47,H=50.28,F=17.94,P均＜0.01).MMP9、MPO及sCD40L识别非钙化斑块的ROC曲线下面积分别为0.854、0.792、0.751,当识别非钙化斑块的临界值分别为510.13、537.82、7.05 μg/L时,其诊断敏感度分别为80％、80％和80％,特异度分别为80％、67％和55％.结论 血清MMP9、MPO和sCD40L水平有助于判断冠状动脉斑块的性质.
目的 探討MMP9、MPO及sCD40L在識彆冠狀動脈斑塊性質中的作用.方法 選取2008年4月至2010年1月阜外心血管病醫院門診胸痛患者118例,根據64排螺鏇CT檢查結果,將CT值＜130 Hu的患者入選為非鈣化斑塊組(71例),CT值≥130 Hu的患者入選為鈣化斑塊組(47例).選取90名健康體檢者為對照組.採用ELISA檢測血清MMP9、MPO及sCD40L水平,併比較其在各組中水平的差異.採用ROC麯線評價各標誌物診斷非鈣化斑塊的敏感度和特異度.結果 非鈣化斑塊組血清MMP9、MPO、sCD40L水平分彆為(762.25±368.71)、[844.10(582.00～ 1220.70)]、(9.37±3.15) μg/L,高于健康對照組的(342.70±178.53)、[426.35(283.20～592.00)]、(6.55±2.96) μg/L及鈣化斑塊組的(483.12±219.09)、[469.00(302.45～723.55)]、(7.24±2.86) μg/L,差異均有統計學意義(統計值分彆為F =42.47,H=50.28,F=17.94,P均＜0.01).MMP9、MPO及sCD40L識彆非鈣化斑塊的ROC麯線下麵積分彆為0.854、0.792、0.751,噹識彆非鈣化斑塊的臨界值分彆為510.13、537.82、7.05 μg/L時,其診斷敏感度分彆為80％、80％和80％,特異度分彆為80％、67％和55％.結論 血清MMP9、MPO和sCD40L水平有助于判斷冠狀動脈斑塊的性質.
목적 탐토MMP9、MPO급sCD40L재식별관상동맥반괴성질중적작용.방법 선취2008년4월지2010년1월부외심혈관병의원문진흉통환자118례,근거64배라선CT검사결과,장CT치＜130 Hu적환자입선위비개화반괴조(71례),CT치≥130 Hu적환자입선위개화반괴조(47례).선취90명건강체검자위대조조.채용ELISA검측혈청MMP9、MPO급sCD40L수평,병비교기재각조중수평적차이.채용ROC곡선평개각표지물진단비개화반괴적민감도화특이도.결과 비개화반괴조혈청MMP9、MPO、sCD40L수평분별위(762.25±368.71)、[844.10(582.00～ 1220.70)]、(9.37±3.15) μg/L,고우건강대조조적(342.70±178.53)、[426.35(283.20～592.00)]、(6.55±2.96) μg/L급개화반괴조적(483.12±219.09)、[469.00(302.45～723.55)]、(7.24±2.86) μg/L,차이균유통계학의의(통계치분별위F =42.47,H=50.28,F=17.94,P균＜0.01).MMP9、MPO급sCD40L식별비개화반괴적ROC곡선하면적분별위0.854、0.792、0.751,당식별비개화반괴적림계치분별위510.13、537.82、7.05 μg/L시,기진단민감도분별위80％、80％화80％,특이도분별위80％、67％화55％.결론 혈청MMP9、MPO화sCD40L수평유조우판단관상동맥반괴적성질.
Objective To evaluate the utility of MMP9,MPO and sCD40L in detection of the character of coronary artery plaque.Methods From April 2008 to January 2010,118 patients from outpatient of Fu Wai Hospital with chest pain were enrolled.All of them underwent 64 Multiple-detector row spiral computer tomography (64-MDCT),the CT value ＜ 130 Hu patients were enrolled in non-calcified plaque group (71 cases),CT value ≥ 130 Hu patients were enrolled in the calcified plaque group (47 cases).Ninty healthy volunteers were selected as the control group.Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of serum markers,including MMP9,MPO and sCD40L.Levels of MMP9,MPO and sCD40L of each group were compared.ROC curve was used to evaluate the sensitivity and specificity of the markers in diagnosis of non-calcified plaque.Results MMP9,MPO and sCD40L levels of non-calcified were ( 762.25 ± 368.71 ),[ 844.10 (582.00 - 1220.70) ],(9.37 ± 3.15) μg/L,higher than the healthy control group (342.70 ± 178.53),[426.35 ( 283.20 - 592.00) ],(6.55 ± 2.96) μg/L and calcified plaque group ( 483.12 ± 219.09 ),[ 469.00 ( 302.45 - 723.55) ],( 7.24 ± 2.86) μg/L The difference was statistically significant ( F =42.47,H =50.28,F =17.94,all P ＜ 0.01 ). Areas of MMP9,MPO and sCD40L under the ROC curve to predict non-calcified plaque were 0.854,0.792,0.751 respectively,when the identification threshold for non-calcified plaque were 510.13,537.82,7.05 μg/L respectively,the diagnostic sensitivity was 80％,80％,80％ respectively,and specificity was 80％,67％ and 55％ respectively.Conclusion The serum MMP9,MPO and sCD40L levels can help to determine the character of coronary plaque.
