CAJ | 학술논문

目的观察藤黄酸(GA)对人肾癌786-O细胞增殖及侵袭能力的影响.方法用不同浓度的GA处理786-O细胞24h后,分别采用噻唑蓝(MTT)比色法、流式细胞技术和Transwell侵袭实验检测GA对肾癌786-O细胞增殖活性和侵袭能力的抑制作用,Western blot检测基质金属蛋白酶(MMP)-9和bax蛋白的表达水平.结果 GA浓度1.5、3.0、6.0μmol/L的实验组能显著抑制786-O细胞的增殖和促进其凋亡.与对照组比较,增殖抑制率分别为(18.45±3.41)％、(27.88±6.24)％、(50.48±12.13)％ (P＜ 0.05)；凋亡率分别为(21.15±1.68)％、(33.57±6.35)％、(46.33±9.74)％(P＜0.05).而GA浓度为0.375、0.750 μmol/L的实验组明显抑制了786-O细胞的侵袭能力,与对照组比较,其侵袭细胞数分别为对照组的(71.50±11.30)％和(52.40±8.20)％(P＜0.05).而且,用浓度为0、1.5、3.0、6.0μmol/L的GA处理过的肾癌786-O细胞,bax蛋白的表达水平明显上调.而当GA浓度为0、0.375、0.750 μmol/L时MMP-9蛋白的表达水平则显著降低.结论 GA不仅对人肾癌786 -O细胞的生长活性具有一定的抑制作用,而且能够有效地抑制其侵袭能力,GA对786-O细胞的这种生物活性的影响可能与bax和MMP-9蛋白的表达有关.
목적 관찰등황산(GA)대인신암786-O세포증식급침습능력적영향.방법 용불동농도적GA처리786-O세포24h후,분별채용새서람(MTT)비색법、류식세포기술화Transwell침습실험검측GA대신암786-O세포증식활성화침습능력적억제작용,Western blot검측기질금속단백매(MMP)-9화bax단백적표체수평.결과 GA농도1.5、3.0、6.0μmol/L적실험조능현저억제786-O세포적증식화촉진기조망.여대조조비교,증식억제솔분별위(18.45±3.41)％、(27.88±6.24)％、(50.48±12.13)％ (P＜ 0.05)；조망솔분별위(21.15±1.68)％、(33.57±6.35)％、(46.33±9.74)％(P＜0.05).이GA농도위0.375、0.750 μmol/L적실험조명현억제료786-O세포적침습능력,여대조조비교,기침습세포수분별위대조조적(71.50±11.30)％화(52.40±8.20)％(P＜0.05).이차,용농도위0、1.5、3.0、6.0μmol/L적GA처리과적신암786-O세포,bax단백적표체수평명현상조.이당GA농도위0、0.375、0.750 μmol/L시MMP-9단백적표체수평칙현저강저.결론 GA불부대인신암786 -O세포적생장활성구유일정적억제작용,이차능구유효지억제기침습능력,GA대786-O세포적저충생물활성적영향가능여bax화MMP-9단백적표체유관.
Objective To explore the effect of gambogic acid (GA) on the growth activity and invasion in renal carcinoma 786-O cells.Methods After treatment with different concentrations of GA for 24 h,the cell viability and invasion ability were measured by methyl thiazol tetrazolium (MTT) assay,flow cytometry and invasion assay,respectively.The protein expression of bax and matrix metalloproteinase ( MMP)-9 were detected by using Western blotting.Results In comparison with controls,the 1.5,3.0 and 6.0 μmol/L GA group could inhibit the cell proliferation,and induce the cell apoptosis.In the 1.5,3.0 and 6.0 μmol/L GA groups,the 1.5,3.0 and 6.0 μmol/L GA group The inhibition rate of cell growth was ( 18.45 ± 3.41 ) ％,( 27.88 ± 6.24) ％,( 50.48 ± 12.13 ) ％ ( P ＜ 0.05 ),and the apoptosis rate was (21.15 ± 1.68 ) ％,( 33.57 ± 6.35 ) ％,(46.33 ± 9.74 ) ％ ( P ＜ 0.05 ) respectively.In addition,the number of invasion cell was decreased significantly after treatment with 0.375 and 0.750 μmol/L GA.Moreover,the expression of bax protein was up-regulated and the MMP-9 was down-regulated in GAtreated groups.Conclusion GA could not only suppress renal cell carcinoma 786-O cell growth but also inhibit the cell invasion,which may be associated with the expression of bax and MMP-9.