中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2009年
1期
48-53
,共6页
陈野野%刘昌伟%叶炜%张然%韦玉生%刘德培
陳野野%劉昌偉%葉煒%張然%韋玉生%劉德培
진야야%류창위%협위%장연%위옥생%류덕배
人工血管%内膜增生%单核细胞趋化蛋白-1%血小板源生长因子-B
人工血管%內膜增生%單覈細胞趨化蛋白-1%血小闆源生長因子-B
인공혈관%내막증생%단핵세포추화단백-1%혈소판원생장인자-B
Blood vessel prosthesis%Tunica intima%Monocyte chemotactic protein-1%Platelet-derived growth factor
目的 研究人工血管旁路术后远端吻合口局部内膜增生的形态学表现、部分炎症因子及生长因子的表达及药物巴曲酶对其的影响.方法 建立12只犬左颈总动脉膨体聚四氟乙烯(eVrrE)人工血管旁路移植模型,随机分为巴曲酶组和对照组,28 d后吻合口局部取材,应用HE、Masson染色、免疫组化(IHC)、逆转录聚合酶链反应(RT-PCR)、免疫印迹(Western Blot)等实验方法 观察吻合口内膜厚度、胞外基质(ECM)相对含量、新生内膜平滑肌细胞增殖率及单核细胞趋化蛋白.1(MCP-1)、血小板源牛长因子B(PDGF-B)mRNA和蛋白表达,并用半定量方法 比较两组间的差异.结果 术后28d,远端吻合口均已形成有内皮细胞(EC)覆盖的新生内膜,且均表达MCP-1及PDGF.B.实验组与对照组相比,其吻合口新生内膜厚度明显减少[(381.3±144.7)μm对(213.8±29.0)μm,P=0.036],胞外基质相对含量明显降低(P=0.006),MCP-1表达量明显下降(RT-PCR P=0.025,Western Blot P=0.016),而PDGF-B表达量差异无统计学意义(RT-PCR P=0.055,WesternBlot P=0.337),平滑肌细胞增殖率无明显差异(P=0.109).结论 巴曲酶可抑制人工血管旁路术后早期吻合口新生内膜胞外基质的沉积和MCP-1的表达,抑制新生内膜厚度,为提高人工血管移植远期通畅率提供新的临床应用及研究思路.
目的 研究人工血管徬路術後遠耑吻閤口跼部內膜增生的形態學錶現、部分炎癥因子及生長因子的錶達及藥物巴麯酶對其的影響.方法 建立12隻犬左頸總動脈膨體聚四氟乙烯(eVrrE)人工血管徬路移植模型,隨機分為巴麯酶組和對照組,28 d後吻閤口跼部取材,應用HE、Masson染色、免疫組化(IHC)、逆轉錄聚閤酶鏈反應(RT-PCR)、免疫印跡(Western Blot)等實驗方法 觀察吻閤口內膜厚度、胞外基質(ECM)相對含量、新生內膜平滑肌細胞增殖率及單覈細胞趨化蛋白.1(MCP-1)、血小闆源牛長因子B(PDGF-B)mRNA和蛋白錶達,併用半定量方法 比較兩組間的差異.結果 術後28d,遠耑吻閤口均已形成有內皮細胞(EC)覆蓋的新生內膜,且均錶達MCP-1及PDGF.B.實驗組與對照組相比,其吻閤口新生內膜厚度明顯減少[(381.3±144.7)μm對(213.8±29.0)μm,P=0.036],胞外基質相對含量明顯降低(P=0.006),MCP-1錶達量明顯下降(RT-PCR P=0.025,Western Blot P=0.016),而PDGF-B錶達量差異無統計學意義(RT-PCR P=0.055,WesternBlot P=0.337),平滑肌細胞增殖率無明顯差異(P=0.109).結論 巴麯酶可抑製人工血管徬路術後早期吻閤口新生內膜胞外基質的沉積和MCP-1的錶達,抑製新生內膜厚度,為提高人工血管移植遠期通暢率提供新的臨床應用及研究思路.
목적 연구인공혈관방로술후원단문합구국부내막증생적형태학표현、부분염증인자급생장인자적표체급약물파곡매대기적영향.방법 건립12지견좌경총동맥팽체취사불을희(eVrrE)인공혈관방로이식모형,수궤분위파곡매조화대조조,28 d후문합구국부취재,응용HE、Masson염색、면역조화(IHC)、역전록취합매련반응(RT-PCR)、면역인적(Western Blot)등실험방법 관찰문합구내막후도、포외기질(ECM)상대함량、신생내막평활기세포증식솔급단핵세포추화단백.1(MCP-1)、혈소판원우장인자B(PDGF-B)mRNA화단백표체,병용반정량방법 비교량조간적차이.결과 술후28d,원단문합구균이형성유내피세포(EC)복개적신생내막,차균표체MCP-1급PDGF.B.실험조여대조조상비,기문합구신생내막후도명현감소[(381.3±144.7)μm대(213.8±29.0)μm,P=0.036],포외기질상대함량명현강저(P=0.006),MCP-1표체량명현하강(RT-PCR P=0.025,Western Blot P=0.016),이PDGF-B표체량차이무통계학의의(RT-PCR P=0.055,WesternBlot P=0.337),평활기세포증식솔무명현차이(P=0.109).결론 파곡매가억제인공혈관방로술후조기문합구신생내막포외기질적침적화MCP-1적표체,억제신생내막후도,위제고인공혈관이식원기통창솔제공신적림상응용급연구사로.
Objective To study the morphological behavior ,the expression of MCP-1 and PDGF-B of distal anastomotic neointima after expanded polytetrafluoroethylene (ePTFE) bypass grafting. And to identify the effects of Batroxobin (BX) on distal anastomotic intimal hyperplasia. Methods 12 adult mongrel dogs underwent end to side bypass grafting in left common carotid artery using ePTFE vascular graft (6mm in diameter, 5cm in length) were divided randomly into two groups. All 12 bypass grafts were removed 28 days after grafting and distal anastomotic parts were obtained . Using HE, Masson , IHC, RT-PCR and western blot , we analyzed the thickness, relative content of ECM, SMC PCNA index, expression of MCP-1 and PDGF-B in distal anastomotic neointima. And we compared the differences between the two groups. Results 28 day after grafting , the neointima was formed by SMC and ECM and was covered by epithelial cells. After using BX , the thickness of neointima was reduced [(381.3±144.7) μm versus (213.8±29.0) μm, P <0.05] and the relative content of ECM were significantly decreased(P =0.006). The PCNA index(P = 0.109) and PDGF-B level (P =0.055 by RT-PCR and P = 0.337 by WB) between the two groups had no significant difference . MCP-1 level was markedly decreased (P = 0.025 by RT-PCR and P = 0.016 by WB). Conclusions Using BX after ePTFE grafting could effectively reduce the relative content of ECM ,rnduce the expression of MCP-1 and decrease the thickness of neointima in the early time.
