CAJ | 학술논문

[目的]研究利用酶解法提取竹笋不溶性膳食纤维.[方法]采用正交试验设计对竹笋不溶性膳食纤维的提取条件进行了研究.[结果]各因素对竹笋不溶性膳食纤维提取影响程度依次为:α-淀粉酶>酶解时间>木瓜蛋白酶>pH值>料水比>纤维素酶>酶解温度;竹笋不溶性膳食纤维提取条件的最佳组合为:料水比1∶40,α-淀粉酶1 600 U/g底物,木瓜蛋白酶3 000 U/g底物,纤维素酶4 000 U/g底物,pH值5.0,酶解温度55 ℃,酶解时间1.5 h.[结论]筛选出了影响膳食纤维提取的主要影响因素,得到了竹笋膳食纤维酶解法的最佳条件,为进一步改良和优化膳食纤维的成分和生理功能提供了科学依据.
[목적]연구이용매해법제취죽순불용성선식섬유.[방법]채용정교시험설계대죽순불용성선식섬유적제취조건진행료연구.[결과]각인소대죽순불용성선식섬유제취영향정도의차위:α-정분매>매해시간>목과단백매>pH치>료수비>섬유소매>매해온도;죽순불용성선식섬유제취조건적최가조합위:료수비1∶40,α-정분매1 600 U/g저물,목과단백매3 000 U/g저물,섬유소매4 000 U/g저물,pH치5.0,매해온도55 ℃,매해시간1.5 h.[결론]사선출료영향선식섬유제취적주요영향인소,득도료죽순선식섬유매해법적최가조건,위진일보개량화우화선식섬유적성분화생리공능제공료과학의거.
[Objective] The research aimed to study the extraction of insoluble dietary fiber(IDF) from bamboo shoot basis on enzymolysis method. [Method] The orthogonal design method was used to study the extraction conditions of bamboo shoot IDF. [Result] The results showed that the influence degree of different factors to extraction of bamboo shoot IDF were in order: α-amylase>enzymolysis time>papain> pH value> ratio of material to water> cellulase> enzymolysis temperature. The best combination about extraction conditions of bamboo shoot IDF was as follow: ratio of material to water 1∶40, α-amylase 1 600 U/g(substrate), papain 3 000 U/g(substrate), cellulase 4 000 U/g(substrate), pH value 5.0, enzymolysis temperature 55 ℃, enzymolysis time 1.5 h. [Conclusion] The study screens out the main influence factors of extracting IDF from bamboo shoot, and gets the best conditions of extracting IDF from bamboo shoot by enzymolysis method. It can provide the scientific basis for deeply improving and optimizing the components and physiological function of IDF.