食品科学
食品科學
식품과학
FOOD SCIENCE
2010年
1期
240-242
,共3页
天麻糖蛋白%快速蛋白液相色谱%急性血瘀%血液流变
天痳糖蛋白%快速蛋白液相色譜%急性血瘀%血液流變
천마당단백%쾌속단백액상색보%급성혈어%혈액류변
glycoprotein from Gastrodia elata%fast protein liquid chromatography%acute blood stasis%hemorrheology
目的:从天麻中分离纯化得到糖蛋白组分GGE2b,研究其对急性血瘀大鼠血液流变学指标的影响.方法:采用离子交换和快速蛋白液相层析色谱(FPLC)技术分离天麻水溶性提取物,得到组分GGE2b.采用皮下注射盐酸肾上腺素结合冷刺激的方法制造急性血瘀大鼠模型.观察GGE2b各剂量组对急性血瘀大鼠全血黏度、血浆黏度、红细胞压积、红细胞聚集指数和红细胞变形指数的影响.结果:离子交换结合FPLC技术分离纯化得到天麻糖蛋白组分GGE2b.GGE2b 60、120mg/kg能够显著降低急性血瘀模型大鼠高切、中切的全血黏度(P<0.05或0.01)和血浆黏度(P<0.01),降低红细胞聚集指数(P<0.05或0.01),增大红细胞变形指数(P<0.05).结论:层析技术用于天麻糖复和物的分离纯化是可行的,且得到的糖蛋白组分GGE2b对急性血瘀大鼠异常的血液流变有良好的治疗效果.
目的:從天痳中分離純化得到糖蛋白組分GGE2b,研究其對急性血瘀大鼠血液流變學指標的影響.方法:採用離子交換和快速蛋白液相層析色譜(FPLC)技術分離天痳水溶性提取物,得到組分GGE2b.採用皮下註射鹽痠腎上腺素結閤冷刺激的方法製造急性血瘀大鼠模型.觀察GGE2b各劑量組對急性血瘀大鼠全血黏度、血漿黏度、紅細胞壓積、紅細胞聚集指數和紅細胞變形指數的影響.結果:離子交換結閤FPLC技術分離純化得到天痳糖蛋白組分GGE2b.GGE2b 60、120mg/kg能夠顯著降低急性血瘀模型大鼠高切、中切的全血黏度(P<0.05或0.01)和血漿黏度(P<0.01),降低紅細胞聚集指數(P<0.05或0.01),增大紅細胞變形指數(P<0.05).結論:層析技術用于天痳糖複和物的分離純化是可行的,且得到的糖蛋白組分GGE2b對急性血瘀大鼠異常的血液流變有良好的治療效果.
목적:종천마중분리순화득도당단백조분GGE2b,연구기대급성혈어대서혈액류변학지표적영향.방법:채용리자교환화쾌속단백액상층석색보(FPLC)기술분리천마수용성제취물,득도조분GGE2b.채용피하주사염산신상선소결합랭자격적방법제조급성혈어대서모형.관찰GGE2b각제량조대급성혈어대서전혈점도、혈장점도、홍세포압적、홍세포취집지수화홍세포변형지수적영향.결과:리자교환결합FPLC기술분리순화득도천마당단백조분GGE2b.GGE2b 60、120mg/kg능구현저강저급성혈어모형대서고절、중절적전혈점도(P<0.05혹0.01)화혈장점도(P<0.01),강저홍세포취집지수(P<0.05혹0.01),증대홍세포변형지수(P<0.05).결론:층석기술용우천마당복화물적분리순화시가행적,차득도적당단백조분GGE2b대급성혈어대서이상적혈액류변유량호적치료효과.
Objective: To isolate and purify glycoprotein from Gastrodia elata (GGE2b) and investigate its hemorrheological effect in acute blood stasis rats. Methods: Soluble extracts of Gastrodia elata were purified by DEAE-Sepharose column chromatography and fast protein liquid chromatography (FPLC) to obtain GGE2b. Acute blood stasis rat model was developed by hypodermic adrenaline injection combined with cold-shock treatment. Totally 60 male rats were randomly divided into 6 groups of 10 rats each, namely saline group, acute blood stasis model group, PGE2b administration groups at doses of 30, 60 and 120 mg/kg, and positive control group administered with Danshen injection. Effect of GGE2b on whole-blood viscosity, plasma viscosity, red blood cell (RBC) accumulation, RBC aggregation index and RBC deformability index in acute blood stasis rats was examined. Results: GGE2b was successfitlly purified from Gastrodia elata through combined technology of DEAE-Sepharose column chromatography and FPLC. Moreover, GGE2b administration at the doses of 60 mg/kg and 120 mg/kg in rats resulted in a dramatic decrease of plasma viscosity (P<0.05), whole-blood viscosity (P<0.01) and RBC aggregation index (P<0.05), and increase of RBC deformability index (P<0.05). Conclusion: It is feasible to purify glycoproteins from Gastrodia elata using chromatography technology. GGE2b can result in a significant improvement of abnormal hemorrheology in rats with acute blood stasis.
