CAJ | 학술논문

研究中药复方制剂连黄(精提、粗提)对大肠埃希菌多重耐药基因AcrA的mRNA表达水平的影响,从而深入探讨中药复方制剂连黄(精提、粗提)抑制大肠埃希菌多重耐药性的作用机理.采用实时荧光定量PCR法,定量分析了中药复方制剂连黄(精提、粗提)作用前后的大肠埃希菌耐药基因AcrA的mRNA表达水平.结果表明,精提和粗提的中药复方制剂均能降低AcrA基因的mRNA表达水平,且精提制剂较粗提制剂对AcrA基因的mRNA表达水平影响更大.
연구중약복방제제련황(정제、조제)대대장애희균다중내약기인AcrA적mRNA표체수평적영향,종이심입탐토중약복방제제련황(정제、조제)억제대장애희균다중내약성적작용궤리.채용실시형광정량PCR법,정량분석료중약복방제제련황(정제、조제)작용전후적대장애희균내약기인AcrA적mRNA표체수평.결과표명,정제화조제적중약복방제제균능강저AcrA기인적mRNA표체수평,차정제제제교조제제제대AcrA기인적mRNA표체수평영향경대.
The influence of Chinese traditional compound medicine on mRNA of Escherichia coli gene AcrA was studied to probe the mechanism of action.The expression of E.coil gene AcrA-mRNA was determined by real-time PCR before and after the using of Chinese traditional compound medicine.The results showed that crude and refined Chinese traditional compound medicine could reduce the expression of AcrA-mRNA,and the refined Chinese traditional compound medicine drug-resistance gene decreased after by dealing with the crude and refined Chinese traditional compound medicine had greater impact on AcrA-mRNA.