白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2011年
9期
522-524
,共3页
黄斯勇%刘利%郝淼旺%何华%谷真%陈任安%秦炜炜%伍艳兰%李国辉%尹郸丹%梁英民
黃斯勇%劉利%郝淼旺%何華%穀真%陳任安%秦煒煒%伍豔蘭%李國輝%尹鄲丹%樑英民
황사용%류리%학묘왕%하화%곡진%진임안%진위위%오염란%리국휘%윤단단%량영민
造血干细胞移植%造血干细胞动员%采集
造血榦細胞移植%造血榦細胞動員%採集
조혈간세포이식%조혈간세포동원%채집
Hematopoietic stem cell transplantation%Hematopoietic stem cell mobilization%Collection
目的 研究外周血造血干细胞移植( PBSCT)供者外周血干细胞动员采集的方法及其效 果。方法 198名健康供者每天皮下注射重组人粒细胞集落刺激因子(rhG-CSF)(5～10) μg/kg进行外周血干细胞动员，第5天开始采集。采用血细胞分析仪行单个核细胞( MNC)计数，流式细胞术(FCM)行CD34+细胞计数。分析供者性别、身高、年龄、采集当天外周血白细胞(WBC)计数对动员采集效果的影响。结果所有供者均成功动员采集，采集当天的MNC计数平均为(4.19±1.96)×108/kg，CD34+细胞计数平均为(2.98±1.40) ×106/kg; MNC和CD34+细胞计数与供者性别、身高、年龄无关；采集当天外周血WBC计数与MNC、CD34+细胞计数呈正相关(r= 0.9201，P=0.0035；r=0.8420，P= 0.0149)；采集当天外周血WBC计数≥20.0×109/L的供者比＜20.0×109/L的供者采集效果更显著(F=4.688，P= 0.0013;F= 4.622，P=0.0006)。结论rhG-CSF动员的健康供者采集当天外周血WBC计数是一项预测CD34+细胞采集数量的简单、可行的指标。
目的 研究外週血造血榦細胞移植( PBSCT)供者外週血榦細胞動員採集的方法及其效 果。方法 198名健康供者每天皮下註射重組人粒細胞集落刺激因子(rhG-CSF)(5～10) μg/kg進行外週血榦細胞動員，第5天開始採集。採用血細胞分析儀行單箇覈細胞( MNC)計數，流式細胞術(FCM)行CD34+細胞計數。分析供者性彆、身高、年齡、採集噹天外週血白細胞(WBC)計數對動員採集效果的影響。結果所有供者均成功動員採集，採集噹天的MNC計數平均為(4.19±1.96)×108/kg，CD34+細胞計數平均為(2.98±1.40) ×106/kg; MNC和CD34+細胞計數與供者性彆、身高、年齡無關；採集噹天外週血WBC計數與MNC、CD34+細胞計數呈正相關(r= 0.9201，P=0.0035；r=0.8420，P= 0.0149)；採集噹天外週血WBC計數≥20.0×109/L的供者比＜20.0×109/L的供者採集效果更顯著(F=4.688，P= 0.0013;F= 4.622，P=0.0006)。結論rhG-CSF動員的健康供者採集噹天外週血WBC計數是一項預測CD34+細胞採集數量的簡單、可行的指標。
목적 연구외주혈조혈간세포이식( PBSCT)공자외주혈간세포동원채집적방법급기효 과。방법 198명건강공자매천피하주사중조인립세포집락자격인자(rhG-CSF)(5～10) μg/kg진행외주혈간세포동원，제5천개시채집。채용혈세포분석의행단개핵세포( MNC)계수，류식세포술(FCM)행CD34+세포계수。분석공자성별、신고、년령、채집당천외주혈백세포(WBC)계수대동원채집효과적영향。결과소유공자균성공동원채집，채집당천적MNC계수평균위(4.19±1.96)×108/kg，CD34+세포계수평균위(2.98±1.40) ×106/kg; MNC화CD34+세포계수여공자성별、신고、년령무관；채집당천외주혈WBC계수여MNC、CD34+세포계수정정상관(r= 0.9201，P=0.0035；r=0.8420，P= 0.0149)；채집당천외주혈WBC계수≥20.0×109/L적공자비＜20.0×109/L적공자채집효과경현저(F=4.688，P= 0.0013;F= 4.622，P=0.0006)。결론rhG-CSF동원적건강공자채집당천외주혈WBC계수시일항예측CD34+세포채집수량적간단、가행적지표。
Objective To investigate the methods and effects on the mobilization and collection of peripheral hematopoietic stem cells. Methods Peripheral hematopoietic stem cells of 198 cases of healthy donors were selected and mobilized by subcutaneous recombinant human granulocyte colony stimulating factor (rhG-CSF) by (5-10) μg·kg-1 ·d-1 and collected on the 5th day. The effects of gender, height, age and WBC count of donors on mobilization and collection were analyzed. Results All the peripheral hematopoietic stem cells of donors were successfully mobilized. The average counts of mononuclear cells (MNC) and CD34+ cells were (4.19±1.96)×108/kg and (2.98±1.40)×106/kg, respectively, which had no correlation with gender, height and age. The counts of MNC and CD34+ cells collected were positively correlated with the WBC count of peripheral blood (r =0.9201, P =0.0035; r =0.8420, P =0.0149). The donors with WBC ≥20.0×109/L had moresignificant effect than those WBC＜20.0×109/L (F =4.688, P =0.0013; F =4.622, P =0.0006). Conclusion The WBC count of peripheral blood from healthy donors is a simple and feasible indicator to predict the quantity of CD34+ cells.
