中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2008年
8期
537-539
,共3页
李晓玲%LIU Xiao-hua%张缪佳%梅焕平%谈文峰
李曉玲%LIU Xiao-hua%張繆佳%梅煥平%談文峰
리효령%LIU Xiao-hua%장무가%매환평%담문봉
红斑狼疮,系统性%间质干细胞%内皮,血管
紅斑狼瘡,繫統性%間質榦細胞%內皮,血管
홍반랑창,계통성%간질간세포%내피,혈관
Lupus erythematosus,systemic%Mesenchymal stem cells%Endothelium,vascular
目的 观察骨髓间质干细胞(MSCs)对系统性红斑狼疮(SLE)患者血清刺激后血管内皮细胞中血管假性血友病因子(vWF)和可溶性血栓调理蛋白(sTM)表达的影响,探讨MSCs对SLE血管内皮损伤后修复的可能作用.方法 人脐静脉内皮细胞株ECV-304和活动期SLE患者血清体外共培养12 h,诱导血管内皮细胞损伤,加入MSCs继续共培养3 d.采用酶联免疫吸附试验(ELISA)法检测上清液中vWF和sTM的表达.结果 SLE血清刺激ECV-304细胞后,上清液中vWF和sTM的表达显著高于未刺激组(P＜0.05)[(30.1±6.1)ng/ml vs(10.2±3.1)ng/ml;(2.41±0.16)vs(1.19±0.14)];加入MSCs后,能显著降低vWF的表达(P＜0.05)[(2.41±0.16)vs(2.19±0.14)],但是对sTM值无明显影响(P＞0.05)[(30.1±6.1)ng/ml vs(26.2±6.0)ng/ml].结论 活动期SLE患者血清可损伤血管内皮细胞,MSCs能降低血管内皮细胞中vWF表达,提示可能对SLE血管内皮损伤有一定的修复作用.
目的 觀察骨髓間質榦細胞(MSCs)對繫統性紅斑狼瘡(SLE)患者血清刺激後血管內皮細胞中血管假性血友病因子(vWF)和可溶性血栓調理蛋白(sTM)錶達的影響,探討MSCs對SLE血管內皮損傷後脩複的可能作用.方法 人臍靜脈內皮細胞株ECV-304和活動期SLE患者血清體外共培養12 h,誘導血管內皮細胞損傷,加入MSCs繼續共培養3 d.採用酶聯免疫吸附試驗(ELISA)法檢測上清液中vWF和sTM的錶達.結果 SLE血清刺激ECV-304細胞後,上清液中vWF和sTM的錶達顯著高于未刺激組(P＜0.05)[(30.1±6.1)ng/ml vs(10.2±3.1)ng/ml;(2.41±0.16)vs(1.19±0.14)];加入MSCs後,能顯著降低vWF的錶達(P＜0.05)[(2.41±0.16)vs(2.19±0.14)],但是對sTM值無明顯影響(P＞0.05)[(30.1±6.1)ng/ml vs(26.2±6.0)ng/ml].結論 活動期SLE患者血清可損傷血管內皮細胞,MSCs能降低血管內皮細胞中vWF錶達,提示可能對SLE血管內皮損傷有一定的脩複作用.
목적 관찰골수간질간세포(MSCs)대계통성홍반랑창(SLE)환자혈청자격후혈관내피세포중혈관가성혈우병인자(vWF)화가용성혈전조리단백(sTM)표체적영향,탐토MSCs대SLE혈관내피손상후수복적가능작용.방법 인제정맥내피세포주ECV-304화활동기SLE환자혈청체외공배양12 h,유도혈관내피세포손상,가입MSCs계속공배양3 d.채용매련면역흡부시험(ELISA)법검측상청액중vWF화sTM적표체.결과 SLE혈청자격ECV-304세포후,상청액중vWF화sTM적표체현저고우미자격조(P＜0.05)[(30.1±6.1)ng/ml vs(10.2±3.1)ng/ml;(2.41±0.16)vs(1.19±0.14)];가입MSCs후,능현저강저vWF적표체(P＜0.05)[(2.41±0.16)vs(2.19±0.14)],단시대sTM치무명현영향(P＞0.05)[(30.1±6.1)ng/ml vs(26.2±6.0)ng/ml].결론 활동기SLE환자혈청가손상혈관내피세포,MSCs능강저혈관내피세포중vWF표체,제시가능대SLE혈관내피손상유일정적수복작용.
Objective Through investigating the effect of mesenchymal stem cells (MSCs) on the expression of yon Willebrand factor (vWF) and soluble thrumbomodulin (sTM) of vascular endothelial cells stimulated by serum of systemic lupus erythematosus (SLE) patients to discuss the repairing effect of MSCs on injured endothelium of SLE patients.Methods When human umbilical vein endothelial cell strain ECV-304 was co-cultivated with serum of SLE patients in vitro for twelve hours in order to induce endothelial cells injury,then MSCs were seeded for three days.Enzyme linked immunosorbent assay (ELISA) was used to detect the expression of vWF and sTM in the supernatant.Results When ECV-304 was stimulated by serum of SLE patients,the expression of vWF and sTM in the supernatant was significantly higher than that in the groups not stimulated by serum of SLE patients;When MSCs were seeded ,the expression of vWF in the Lupus serum stimulated group in which MSCs were seeded was significantly lower than that in the lupus serum stimulated group in which MSCs were not seeded,but the expression of sTM between the lupus serum stimulated group which MSCs were seeded and the lupus serum stimulated group in which MSCs were not seeded was not significantly deviatied.Conclusion Serum of lupus patients at active stage can cause injure vascular endothelial cells.MSCs can downregulate the expression of vWF of vascular endothelial cells.These suggest it may participate in the repairing of injured vascular endothelium of SLE patients.
