CAJ | 학술논문

目的探讨切口痛大鼠脑干嘴端腹内侧髓质谷氨酸和γ-氨基丁酸释放的动态变化.方法雄性SD大鼠,体重250～300 g,麻醉下于右侧脑干嘴端腹内侧髓质内置入微透析导引管.恢复5 d后,选择12只无神经行为学障碍的大鼠,随机分为2组(n=6):A组仅吸入1.2%异氟醚5 min;B组吸入1.2%异氟醚5 min,同时制备右足底切口痛模型.分别于术前(基础状态)、术后3 h、1 d、2 d、3 d时收集微透析液20 μl,采用高压液相法测定微透析液内谷氨酸和γ-氨基丁酸浓度,各神经递质的释放水平以各时点测定值与基础值的百分数表示.结果与基础值比较,B组术后1 d时谷氨酸的释放水平升高(P<0.01),术后2.3 d时谷氨酸的释放水平差异无统计学意义(P>0.05),术后3 h、1 d、2 d.3 d时7.氨基丁酸的释放水平升高(P<0.01);与A组比较,B组术后1 d时谷氨酸的释放水平升高,术后3 h、1 d.2 d.3 d时γ-氨基丁酸的释放水平升高(P<0.01).结论大鼠术后脑干嘴端腹内侧髓质谷氨酸和γ-氨基丁酸的释放增加,可能改变了下行疼痛调控通路的功能.
목적 탐토절구통대서뇌간취단복내측수질곡안산화γ-안기정산석방적동태변화.방법 웅성SD대서,체중250～300 g,마취하우우측뇌간취단복내측수질내치입미투석도인관.회복5 d후,선택12지무신경행위학장애적대서,수궤분위2조(n=6):A조부흡입1.2%이불미5 min;B조흡입1.2%이불미5 min,동시제비우족저절구통모형.분별우술전(기출상태)、술후3 h、1 d、2 d、3 d시수집미투석액20 μl,채용고압액상법측정미투석액내곡안산화γ-안기정산농도,각신경체질적석방수평이각시점측정치여기출치적백분수표시.결과 여기출치비교,B조술후1 d시곡안산적석방수평승고(P<0.01),술후2.3 d시곡안산적석방수평차이무통계학의의(P>0.05),술후3 h、1 d、2 d.3 d시7.안기정산적석방수평승고(P<0.01);여A조비교,B조술후1 d시곡안산적석방수평승고,술후3 h、1 d.2 d.3 d시γ-안기정산적석방수평승고(P<0.01).결론 대서술후뇌간취단복내측수질곡안산화γ-안기정산적석방증가,가능개변료하행동통조공통로적공능.
Objective To investigate the time-dependent changes in glutamate (Glu) and 7-aminobutyric acid ( GABA) release in the rostral ventromedial medulla in a rat model of incisional pain. Methods Healthy male SD rats weighing 250-300 g were used in this study. Twelve rats in which microdialysis cannulae were implanted in the right rostral ventromedial medulla without neurological deficits were randomly divided into 2 groups ( n = 6 each): group A control and group B incisional pain. In group B an 1 cm long incision was made in the plantar surface of right hindpaw under 1.2% isoflurane anesthesia which was maintained for 5 min. Samples of dialysate were collected before incision (T_0 baseline) and at 3 h, 1 d, 2 d and 3 d after incision was made (T_(1-4)) in both groups for determination of Clu and GABA concentrations (by HPLC). Results In group B Glu and GABA concentrations in the dialysate were significantly increased at 1 d (T_2) and 3 h-3 d (T_(1-4)) respectively as compared with the baseline value at T_0 and were significantly higher than those in group A (control group). Conclusion Incisional pain increases the release of Glu and GABA in the rostral ventromedial medulla which might influence the function of descending pain modulation pathway.