CAJ | 학술논문

目的研究三氧化二砷(Arsenic trioxide,ATO)对MRL/lpr小鼠狼疮性肾炎的治疗作用及对肾小管TGF-β1表达的影响.方法将MRL/lpr狼疮鼠45只随机平分为ATO组、CTX组和NS组,观察各组MRL/lpr狼疮小鼠治疗前后尿蛋白(半定量)的变化、肾组织病理改变、肾组织免疫球蛋白IgG、补体C3的表达及用免疫组织化学方法检测肾组织TGF-β1的表达.结果尿蛋白分析:给药后ATO组较NS组有明显减少(P<0.01)、CTX组较NS组也有减少(P<0.01).免疫荧光IgG、补体C3的表达:在NS组可见IgG沿肾小球系膜区,毛细血管袢弥漫沉积(+～3+),而ATO组、CTX组则明显减少(-～+),经统计发现ATO组、CTX组较Ns组明显减少(P<0.05);补体C3的表达差异无统计学意义.肾小球细胞数及肾组织活动积分在ATO组及CTX组中均较NS组明显减少(P<0.05).免疫组化:TGF-β1在肾小管可见明显表达,肾小球表达不明显,ATO组的阳性小管数较NS组明显减少(20.28 4±1.90对68.23±2.87,P<0.01),CTX组的阳性小管数较NS组明显减少(23.26±1.71对68.23±2.87,P<0.01).结论三氧化二砷可以下调肾组织TGF-β1的表达改善小管病变,同时可以通过缓解肾小球、小管间质炎症及减少免疫复合物的沉积达到改善狼疮性肾炎的进展.
목적 연구삼양화이신(Arsenic trioxide,ATO)대MRL/lpr소서랑창성신염적치료작용급대신소관TGF-β1표체적영향.방법 장MRL/lpr랑창서45지수궤평분위ATO조、CTX조화NS조,관찰각조MRL/lpr랑창소서치료전후뇨단백(반정량)적변화、신조직병리개변、신조직면역구단백IgG、보체C3적표체급용면역조직화학방법검측신조직TGF-β1적표체.결과 뇨단백분석:급약후ATO조교NS조유명현감소(P<0.01)、CTX조교NS조야유감소(P<0.01).면역형광IgG、보체C3적표체:재NS조가견IgG연신소구계막구,모세혈관번미만침적(+～3+),이ATO조、CTX조칙명현감소(-～+),경통계발현ATO조、CTX조교Ns조명현감소(P<0.05);보체C3적표체차이무통계학의의.신소구세포수급신조직활동적분재ATO조급CTX조중균교NS조명현감소(P<0.05).면역조화:TGF-β1재신소관가견명현표체,신소구표체불명현,ATO조적양성소관수교NS조명현감소(20.28 4±1.90대68.23±2.87,P<0.01),CTX조적양성소관수교NS조명현감소(23.26±1.71대68.23±2.87,P<0.01).결론 삼양화이신가이하조신조직TGF-β1적표체개선소관병변,동시가이통과완해신소구、소관간질염증급감소면역복합물적침적체도개선랑창성신염적진전.
Objective To investigate the roles of Arsenic Trioxide to the lupus nephritis in MRL/ lpr mice and the effect on the expression of TGF-pl in the renal tubule. Methods 45 MRL/lpr mice were chosen for such experiment, then separated in 3 different groups, including arsenic trioxide ( ATO) group, cyclophosphamide (CYC) group and sodium chloride (NS) group. Urine of these mice was kept after the treatment to detect the urine protein levels. The pathological changes and the expression of IgG were observed , and the complement 3 of the nephridial tissue as well as the TGF-pl were detected by immunohisto-chemistry. Result The levels of urine protein in ATO group and CTX group were lower than NS group after the treatment ( P <0.05). The expression of IgG along the glomerular mesangium and capillary loop in ATO and CTX groups was less than NS group ( P <0.05). The expression of C3 had no significant difference within three groups ( P > 0.05). The glomcrulus spherulous cells and the integral of activity in ATO group and CTX group were less than that in NS group ( P <0.05). TGF-β1 wildly presented in the epithelial cells endochylema of the nephric tubule, but it was rarely seen in glomcrulus in NS group. The expression in the nephric tubule of ATO group was less than NS group (20.28 ± 1. 90 vs 68. 23 ± 2. 87, P < 0.01) , and the expression in the nephric tubule in CTX group was less than that in NS group (23. 26 ± 1.71 vs 68. 23 ±2. 87, P <0. 01). Conclusion TGF-pl may take part in the immunopathogenesis of lupus nephritis in MRI/lpr mice. ATO can relieve the inflammation of nephric tubule by down regulation the expression of TGF-β1. ATO can also relieve the tissue damage of kidney in lupus nephritis through reducing the inflammation of glomcrulus and interstitial substance,as well as the expression of the immunocomplex.