中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2010年
4期
328-331
,共4页
骨化三醇%周围神经%运动神经元%脊髓
骨化三醇%週圍神經%運動神經元%脊髓
골화삼순%주위신경%운동신경원%척수
Calcitriol%Peripheral nerves%Motor neurons%Spinal cord
目的 探讨骨化三醇[1,25(OH)_2D_3]对周围神经损伤后脊髓前角运动神经元的保护作用. 方法 48只SD大鼠随机分为安慰剂组和骨化三醇组,每组24只,切断右侧坐骨神经,近端结扎,远端埋入股二头肌.术后骨化三醇组给予骨化三醇2μg·kg~(-1)·d~(-1)灌胃,安慰剂组给予脂肪乳剂2 ml·kg~(-1)·d~(-1)灌胃,灌胃7 d.术后1、2、4周分别处死动物,取腰膨大(L4～L6节段)脊髓,分别行尼氏染色,胆碱酯酶(CHE)染色和酸性磷酸酶(ACP)染色,并结合计算机进行图像分析. 结果术后1、2、4周,骨化三醇组脊髓前角运动神经元存活率分别为(90.8±3.5)%、(84.1±2.3)%、(76.2±2.7)%,高于安慰剂组((84.3±6.5)%、(76.3±2.7)%、(73.4±1.9)%](t=-5.442、-6.443、-2.286,P<0.01或P<0.05);骨化三醇组脊髓CHE染色阳性面积百分率分别为(69.1±1.4)%、(74.5±3.4)%、(78.9±6.6)%,高于安慰剂组[(59.5±1.0)%、(65.8±4.0)%、(66.8±5.3)%](t=-15.787、-10.781、-11.954,P<0.01);骨化三醇组脊髓ACP染色阳性面积百分率分别为(182.8±5.5)%、(115.0±12.5)%、(108.7±8.4)%,低于安慰剂组[(206.5±14.7)%、(138.2±9.9)%、(115.1±9.0)%],第1、2周t=4.275、4.113,P<0.01;第4周t=1.458,P=0.167. 结论骨化三醇对周围神经损伤所致的脊髓前角运动神经元损伤有一定的保护作用.
目的 探討骨化三醇[1,25(OH)_2D_3]對週圍神經損傷後脊髓前角運動神經元的保護作用. 方法 48隻SD大鼠隨機分為安慰劑組和骨化三醇組,每組24隻,切斷右側坐骨神經,近耑結扎,遠耑埋入股二頭肌.術後骨化三醇組給予骨化三醇2μg·kg~(-1)·d~(-1)灌胃,安慰劑組給予脂肪乳劑2 ml·kg~(-1)·d~(-1)灌胃,灌胃7 d.術後1、2、4週分彆處死動物,取腰膨大(L4～L6節段)脊髓,分彆行尼氏染色,膽堿酯酶(CHE)染色和痠性燐痠酶(ACP)染色,併結閤計算機進行圖像分析. 結果術後1、2、4週,骨化三醇組脊髓前角運動神經元存活率分彆為(90.8±3.5)%、(84.1±2.3)%、(76.2±2.7)%,高于安慰劑組((84.3±6.5)%、(76.3±2.7)%、(73.4±1.9)%](t=-5.442、-6.443、-2.286,P<0.01或P<0.05);骨化三醇組脊髓CHE染色暘性麵積百分率分彆為(69.1±1.4)%、(74.5±3.4)%、(78.9±6.6)%,高于安慰劑組[(59.5±1.0)%、(65.8±4.0)%、(66.8±5.3)%](t=-15.787、-10.781、-11.954,P<0.01);骨化三醇組脊髓ACP染色暘性麵積百分率分彆為(182.8±5.5)%、(115.0±12.5)%、(108.7±8.4)%,低于安慰劑組[(206.5±14.7)%、(138.2±9.9)%、(115.1±9.0)%],第1、2週t=4.275、4.113,P<0.01;第4週t=1.458,P=0.167. 結論骨化三醇對週圍神經損傷所緻的脊髓前角運動神經元損傷有一定的保護作用.
목적 탐토골화삼순[1,25(OH)_2D_3]대주위신경손상후척수전각운동신경원적보호작용. 방법 48지SD대서수궤분위안위제조화골화삼순조,매조24지,절단우측좌골신경,근단결찰,원단매입고이두기.술후골화삼순조급여골화삼순2μg·kg~(-1)·d~(-1)관위,안위제조급여지방유제2 ml·kg~(-1)·d~(-1)관위,관위7 d.술후1、2、4주분별처사동물,취요팽대(L4～L6절단)척수,분별행니씨염색,담감지매(CHE)염색화산성린산매(ACP)염색,병결합계산궤진행도상분석. 결과술후1、2、4주,골화삼순조척수전각운동신경원존활솔분별위(90.8±3.5)%、(84.1±2.3)%、(76.2±2.7)%,고우안위제조((84.3±6.5)%、(76.3±2.7)%、(73.4±1.9)%](t=-5.442、-6.443、-2.286,P<0.01혹P<0.05);골화삼순조척수CHE염색양성면적백분솔분별위(69.1±1.4)%、(74.5±3.4)%、(78.9±6.6)%,고우안위제조[(59.5±1.0)%、(65.8±4.0)%、(66.8±5.3)%](t=-15.787、-10.781、-11.954,P<0.01);골화삼순조척수ACP염색양성면적백분솔분별위(182.8±5.5)%、(115.0±12.5)%、(108.7±8.4)%,저우안위제조[(206.5±14.7)%、(138.2±9.9)%、(115.1±9.0)%],제1、2주t=4.275、4.113,P<0.01;제4주t=1.458,P=0.167. 결론골화삼순대주위신경손상소치적척수전각운동신경원손상유일정적보호작용.
Objective To investigate the protective effect of calcitriol on anterior horn motor neuron of spinal cord after peripheral nerve injury. Methods A total of 48 SD rats (250-300 g body weight) were divided into 2 groups: control group and caleitriol intervention group (n = 24, respectively). The right nervi ischiadicus of rats were cut off with proximal ligation, and the distal ends were embedded into biceps femoris. After surgery, rats were treated with equivalent fat milk (2 ml·kg~(-1)·d~(-1)) in control group, lasting 7 days. And the rats were treated with calcitriol (2 μg · kg~(-1)·d~(-1)) in intervention group. Rats were killed at 1, 2 and 4 weeks. The specimens of L4～L6 spinal cord were taken and sectioned. The Nissl's, cholinesterase (CHE) and acid phosphatase (ACP) staining in motor neurons were executed and analyzed. Results At the same time point, the viabilities of spinal cord anterior horn motor neurons were higher in calcitriol intervention group than in control group [1 week: (90.8±3.5)% vs. (84.3±6.5)(t=-5.442,P<0.01)%; 2 weeks: (84.1±2.3)% vs. (76.3±2.7)%(t=-6.443,P<0.01); 4 weeks: (76.2±2.7)% vs. (73.4± 1.9) %] (t=-2.286, P<0.05). The CHE activities were significantly higher in calcitriol intervention group than in control group [1 week: (69.1±1.4)% vs. (59.5±1.0)% (t=-15.787,P<0.01); 2 weeks: (74.5±3.4)% vs. (65.8±4.0)%(t=-10.781,P<0.01); 4 weeks: (78.9±6.6)% vs. (66.8±5.3)% (t=-11.954, P<0.01), and the ACP activities were significantly lower in calcitriol intervention group than in control group (1 week: (182.8±5.5)% vs. (206.5±14.7)%(t=4.275, P<0.01); 2weeks: (115.0±12.5)% vs. (138.2±9.9)%(t=4.113,P<0.01); 4 weeks: (108.7 ±8.4)% vs. (115.1±9.0)%](t=1.458,P<0.16). Conclusions Calcitriol has protective effect on spinal motor neuron after peripheral nerve injury.
