CAJ | 학술논문

目的探讨1,2-二氯乙烷(1,2-DCE)对体外培养细胞周期、凋亡和增殖能力的影响.方法不同浓度1,2-DCE染毒0.5或1h,噻唑蓝(MTT)法检测活细胞相对数和相对活力,流式细胞术(FCM)分析细胞周期和凋亡情况.结果 MTT法检测发现,随1,2-DCE染毒剂量的增加和染毒时间的延长,细胞相对存活率逐渐降低.与二甲亚砜(DMSO)组比较,染毒0.5 h,25、75、100、125、150、175、200 μmol/L 1,2-DCE组的细胞相对存活率降低,差异均有统计学意义(P＜0.05或P＜0.01)；染毒1h,75、100、125、150、175、200 μmol/L 1,2-DCE组的细胞相对存活率降低,差异均有统计学意义(P＜0.05或P＜0.01)；与0.5 h各组比,175μmol/L组染毒1h的细胞相对存活率降低,差异有统计学意义(P＜0.01)；增殖曲线经标准化拟合后发现,染毒0.5 h,1,2-DCE的最适IC50为89.41 μmol/L,95％的可信区间为85.23～93.79μmol/L;染毒1h,1,2-DCE的最适IC50为87.68 μmol/L,95％的可信区间为83.71～91.82 μmol/L.FCM法检测发现,与对照组比较,1,2-DCE染毒1h,25、50、100、150、200 μmol/L组的G0/G1期比例降低,25、50、100 μmol/L组的S期比例降低,25、50、100、150、200 μmol/L组的G2/M期比例升高,差异均有统计学意义(P＜0.05或P＜0.01)；但各组均不引起细胞凋亡.结论 1,2-DCE能够抑制体外培养SW620细胞增殖,使细胞周期停滞在G2/M期,但不诱导细胞凋亡.
목적 탐토1,2-이록을완(1,2-DCE)대체외배양세포주기、조망화증식능력적영향.방법 불동농도1,2-DCE염독0.5혹1h,새서람(MTT)법검측활세포상대수화상대활력,류식세포술(FCM)분석세포주기화조망정황.결과 MTT법검측발현,수1,2-DCE염독제량적증가화염독시간적연장,세포상대존활솔축점강저.여이갑아풍(DMSO)조비교,염독0.5 h,25、75、100、125、150、175、200 μmol/L 1,2-DCE조적세포상대존활솔강저,차이균유통계학의의(P＜0.05혹P＜0.01)；염독1h,75、100、125、150、175、200 μmol/L 1,2-DCE조적세포상대존활솔강저,차이균유통계학의의(P＜0.05혹P＜0.01)；여0.5 h각조비,175μmol/L조염독1h적세포상대존활솔강저,차이유통계학의의(P＜0.01)；증식곡선경표준화의합후발현,염독0.5 h,1,2-DCE적최괄IC50위89.41 μmol/L,95％적가신구간위85.23～93.79μmol/L;염독1h,1,2-DCE적최괄IC50위87.68 μmol/L,95％적가신구간위83.71～91.82 μmol/L.FCM법검측발현,여대조조비교,1,2-DCE염독1h,25、50、100、150、200 μmol/L조적G0/G1기비례강저,25、50、100 μmol/L조적S기비례강저,25、50、100、150、200 μmol/L조적G2/M기비례승고,차이균유통계학의의(P＜0.05혹P＜0.01)；단각조균불인기세포조망.결론 1,2-DCE능구억제체외배양SW620세포증식,사세포주기정체재G2/M기,단불유도세포조망.
Objective To explore the effects of 1,2-dichloroethane (1,2-DCE) on the cellular proliferation,cellular cycle and apoptosis of SW620 cells in vitro.Methods SW620 cells were exposed to 1,2-DCE at different concentrations for 0.5 and 1 h.MTT assay was used to detect the relative number and relative viability,the low cytometry (FCM) assay was utilized to measure the cell cycle and apoptosis.Results The results of MTT assay showed that the cellular relative viability decreased with the 1,2-DCE' s dose and exposure time.Compared with the DMSO group,the relative cellular viability of cells exposed to 1,2-DCE at the doses of 75,100,125,150,175,200 μmol/L for 1 h decreased (P＜0.05 or P＜0.01 ).Compared with the groups exposed to 1,2-DCE for 0.5 h,the relative cellular viability of cells exposed to 175 μmol/L 1,2-DCE for 1 h decreased significantly (P＜0.01).IC50 of cellular proliferation in cells exposed to 1,2-DCE for 0.5 h was 89.41 μ mol/L,and 95％ confidence interval was 85.23 to 93.79 μmol/L.IC50 of cellular proliferation in cells exposed to 1,2-DCE for 1 h was 87.68 μmol/L,and 95％ confidence interval was 83.71 to 91.82 μmol/L.The results of FCM indicated that compared with the control group,the G0/G1 phase in groups exposed to 1,2-DCE at the doses of 25,50,100,150 and 200 μmol/L for 1 h decreased significantly (P＜0.05 or P＜0.01),the S phase in groups exposed to 1,2-DCE at the doses of 25,50 and 100 μ mol/L for 1 h reduced significantly (P＜0.05 or P＜0.01),the G2/M phase in groups exposed to 1,2-DCE at the doses of 25,50,100,150 and 200 μmol/L for 1 h increased significantly (P＜0.05 or P＜0.01 ).However,1,2-DCE could not induce apoptosis of SW620 cells.Conclusion 1,2-DCE could inhibit the proliferation of SW620 cells,and angst SW620 cells at G2/M phase,but could not induce the apoptosis of SW620 cells in vitro.