中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2009年
3期
209-213
,共5页
孙蓬明%魏丽惠%赵丽君%刘宁%王建六%宋一一%陈贤璟%林灏
孫蓬明%魏麗惠%趙麗君%劉寧%王建六%宋一一%陳賢璟%林灝
손봉명%위려혜%조려군%류저%왕건륙%송일일%진현경%림호
子宫内膜肿瘤%转录因子%受体%雌激素
子宮內膜腫瘤%轉錄因子%受體%雌激素
자궁내막종류%전록인자%수체%자격소
Endometrial neoplasms%Transcription factors%Receptors,estrogen
目的 分析并比较不同雌激素受体(ER)表达状态的子宫内膜癌细胞中转录因子的活 性.方法 采用实时荧光定量RT-PCR技术检测子宫内膜癌细胞系RL-952[Erα、Erβ表达均阳性(+)]、HEC-1A[Erα表达弱阳性(±)、Erβ表达阴性(-)]、HEC-1B[Erα、Erβ表达均(-)]细胞中Erα mRNA的表达.采用345通量转录因子芯片检测RL-952、HEC-1A、HEC-1B细胞中转录因子的活性,采用酶联免疫吸附试验(ELISA)方法检测不同转录活性的转录因子NFkKBp65、p38MAPK以验证芯片检测结果.结果 Erα mRNA在RL-952、HEC-1A、HEC-1B细胞中表达水平依次递减,分别为(6780±282)、(684±84)、(168±38)copy/ng.转录因子NFkBp65、p38MAPK的转录活性采用ELISA方法检测(分别为2.0±0.4、0.9±0.5,P=0.020)与芯片检测(分别为3003±530、882±538,P:0.017)结果一致.在345个转录因子中,筛选出与ER功能相关的差异表达的转录因子共28个,与ER(+)的RL-952细胞相比,ER(-)的HEC-1A、HEC-1B细胞中转录因子活性同时上调的有13种,同时下调的有15种.转录因子TTF(1)-1、NRF-1、TCE的活性与Erα mRNA表达水平呈明显线性正相关关系(r=0.523,P=0.037),而转录因子RFX123和Ikaros的活性与Erα mRNA表达水平呈明显非线性负相关关系(r=-0.312,P=0.041).结论转录因子芯片检测是筛选子宫内膜癌致病机制中主要转录因子的先进技术.转录因子TTF(1)-1、NRF-1、TCE可能与ER(+)子宫内膜癌中的信号传导通路相关;转录因子RFX123和Ikams可能与ER(-)子宫内膜癌中的信号传导通路相关.
目的 分析併比較不同雌激素受體(ER)錶達狀態的子宮內膜癌細胞中轉錄因子的活 性.方法 採用實時熒光定量RT-PCR技術檢測子宮內膜癌細胞繫RL-952[Erα、Erβ錶達均暘性(+)]、HEC-1A[Erα錶達弱暘性(±)、Erβ錶達陰性(-)]、HEC-1B[Erα、Erβ錶達均(-)]細胞中Erα mRNA的錶達.採用345通量轉錄因子芯片檢測RL-952、HEC-1A、HEC-1B細胞中轉錄因子的活性,採用酶聯免疫吸附試驗(ELISA)方法檢測不同轉錄活性的轉錄因子NFkKBp65、p38MAPK以驗證芯片檢測結果.結果 Erα mRNA在RL-952、HEC-1A、HEC-1B細胞中錶達水平依次遞減,分彆為(6780±282)、(684±84)、(168±38)copy/ng.轉錄因子NFkBp65、p38MAPK的轉錄活性採用ELISA方法檢測(分彆為2.0±0.4、0.9±0.5,P=0.020)與芯片檢測(分彆為3003±530、882±538,P:0.017)結果一緻.在345箇轉錄因子中,篩選齣與ER功能相關的差異錶達的轉錄因子共28箇,與ER(+)的RL-952細胞相比,ER(-)的HEC-1A、HEC-1B細胞中轉錄因子活性同時上調的有13種,同時下調的有15種.轉錄因子TTF(1)-1、NRF-1、TCE的活性與Erα mRNA錶達水平呈明顯線性正相關關繫(r=0.523,P=0.037),而轉錄因子RFX123和Ikaros的活性與Erα mRNA錶達水平呈明顯非線性負相關關繫(r=-0.312,P=0.041).結論轉錄因子芯片檢測是篩選子宮內膜癌緻病機製中主要轉錄因子的先進技術.轉錄因子TTF(1)-1、NRF-1、TCE可能與ER(+)子宮內膜癌中的信號傳導通路相關;轉錄因子RFX123和Ikams可能與ER(-)子宮內膜癌中的信號傳導通路相關.
목적 분석병비교불동자격소수체(ER)표체상태적자궁내막암세포중전록인자적활 성.방법 채용실시형광정량RT-PCR기술검측자궁내막암세포계RL-952[Erα、Erβ표체균양성(+)]、HEC-1A[Erα표체약양성(±)、Erβ표체음성(-)]、HEC-1B[Erα、Erβ표체균(-)]세포중Erα mRNA적표체.채용345통량전록인자심편검측RL-952、HEC-1A、HEC-1B세포중전록인자적활성,채용매련면역흡부시험(ELISA)방법검측불동전록활성적전록인자NFkKBp65、p38MAPK이험증심편검측결과.결과 Erα mRNA재RL-952、HEC-1A、HEC-1B세포중표체수평의차체감,분별위(6780±282)、(684±84)、(168±38)copy/ng.전록인자NFkBp65、p38MAPK적전록활성채용ELISA방법검측(분별위2.0±0.4、0.9±0.5,P=0.020)여심편검측(분별위3003±530、882±538,P:0.017)결과일치.재345개전록인자중,사선출여ER공능상관적차이표체적전록인자공28개,여ER(+)적RL-952세포상비,ER(-)적HEC-1A、HEC-1B세포중전록인자활성동시상조적유13충,동시하조적유15충.전록인자TTF(1)-1、NRF-1、TCE적활성여Erα mRNA표체수평정명현선성정상관관계(r=0.523,P=0.037),이전록인자RFX123화Ikaros적활성여Erα mRNA표체수평정명현비선성부상관관계(r=-0.312,P=0.041).결론전록인자심편검측시사선자궁내막암치병궤제중주요전록인자적선진기술.전록인자TTF(1)-1、NRF-1、TCE가능여ER(+)자궁내막암중적신호전도통로상관;전록인자RFX123화Ikams가능여ER(-)자궁내막암중적신호전도통로상관.
Objective To analysis the activity of transcriptional factors in endometrial cancer cell lines with different estrogen receptor subtypes. Methods The mRNA levels of estrogen receptor (ER) was detected by quantitative RT-PCR , and the activity of transcriptional factors was also analysed by 345-channel protein/DNA array in RL-952 ( the expression status of ERα and ERβ both positive), HEC-1A [ERα(±),while ERβ negative] and HEC-1B (ERα and ERβ both negative). The transcription factors of NFkBp65 and p38MAPK with different activity were tested by enzyme-linked immunosorbent assay(ELISA) to confirm the results of protein/DNA array. Results The mRNA levels of ERα in RL-952, HEC-1A and HEC-1B were (6780±282 ), ( 684±84 ) and ( 168±38 ) eopy/ng, respectively. Among 345 candidate transcriptional factors, there were 28 factors associated with ER status. Compared with RL-952 cells, 13 transcriptional activity factors were concomitandy up-regulation, while 15 concomitantly down-regulation in HEC-1A and HEC-1B cells. Transcriptional activities of TrF (1)-1, NRF-1, TCE were significantly correlated with the high-expression status of ERα mRNA ( r =0.523, P=0.037 ), while RFX123 and Ikaros were signitleanfly correlated with the low-expression status of ERα mRNA ( r=-0.312, P=0.041 ). Conclusion Transcriptional factors of TTF(1)-1, NBF-1, TCE may be associated with ER-mediated signal pathway, while RFX123 and Ikaros may be associated with non ER-mediatecl signal pathway in endometrial cancer.
