CAJ | 학술논문

目的探讨幽门螺杆菌(Hp)感染与AGS细胞内活性氧类物质(ROS)的关系.方法将Hp11638(CagA~+,VacA~+)菌株及其突变株Hp11638M(CagA~+,VacA~-)提取液与AGS细胞共同孵育,收集细胞及培养上清液.流式细胞术检测细胞内ROS水平,分光光度计550 nm处检测培养上清液中细胞色素C含量.结果 AGS细胞内ROS水平与2株Hp提取液呈浓度及时间依赖关系,经Hp11638提取液刺激的AGS细胞内ROS水平高于Hp11638M提取液.细胞色素C减少证实了这一结果 .结论 AGS细胞内ROS升高是Hp多种蛋白共同作用的结果 ,VacA蛋白参与了这一过程.
목적 탐토유문라간균(Hp)감염여AGS세포내활성양류물질(ROS)적관계.방법 장Hp11638(CagA~+,VacA~+)균주급기돌변주Hp11638M(CagA~+,VacA~-)제취액여AGS세포공동부육,수집세포급배양상청액.류식세포술검측세포내ROS수평,분광광도계550 nm처검측배양상청액중세포색소C함량.결과 AGS세포내ROS수평여2주Hp제취액정농도급시간의뢰관계,경Hp11638제취액자격적AGS세포내ROS수평고우Hp11638M제취액.세포색소C감소증실료저일결과 .결론 AGS세포내ROS승고시Hp다충단백공동작용적결과 ,VacA단백삼여료저일과정.
Objective To investigate the relationship between Helicobacter pylori (H. pylori) infection and concentration of reactive oxygen species (ROS) in AGS cells. Methods AGS cells were cultured with either Hp11638 (CagA~+ , VacA~+ ) extract or Hp11638 mutant (CagA~+ , VacA~-) extract for 48 hours, then the cells and supernatants were collected. The concentration of ROS in AGS cells was measured by flow cytometry. The eytochrome C reduction was detected by spectrophotometer at 550 nm. Results The ROS levels in the AGS cells were correlated with two H. pylori strains in a concentration- and time-dependent manner. The ROS levels in AGS cells treated with Hp11638 extract in different concentrations or times were correspondingly higher than those treated with Hp11638 mutant extract. Similar results were found in examination of cytochrome C reduction. Conclusion The elevation of ROS in AGS cells is related to effects of H. pylori proteins, and the VaeA protein involves in the process.