CAJ | 학술논문

目的筛选家族性腺瘤性息肉病(FAP)的特异表达蛋白,构建判别FAP与散发性肠腺瘤的血清蛋白指纹图谱诊断模型.方法采集19例FAP和16例散发性肠腺瘤患者的血清,以表面增强激光解吸电离飞行时间质谱仪(SELDI-MS-TOF)和阴离子CM01蛋白质芯片检测并筛选两组对象间的血清差异表达蛋白质峰,以支持向量机方法构建判别模型.结果 FAP与散发性肠腺瘤相比,P<0.01的蛋白质峰有6个,其中质荷比为5640、3160、4180和4290的蛋白质峰在FAP中高表达,质荷比为3940和3400的蛋白质峰在散发性肠腺瘤患者中高表达.以质倚比分别为5640、3160和4290的蛋白质峰为基础,联合质荷比为3940、13 750和4300的蛋白质峰所建立的模型判别效果最佳,对FAP与散发性肠腺瘤的判别准确率分别为94.7%和93.7%.结论 SELDI-TOF-MS能有效筛选FAP与散发性肠腺瘤的差异表达蛋白,支持向量机方法所建立的质谱模型判别效果较好,为进一步研究FAP的分子发病机制提供了切入点.
목적 사선가족성선류성식육병(FAP)적특이표체단백,구건판별FAP여산발성장선류적혈청단백지문도보진단모형.방법 채집19례FAP화16례산발성장선류환자적혈청,이표면증강격광해흡전리비행시간질보의(SELDI-MS-TOF)화음리자CM01단백질심편검측병사선량조대상간적혈청차이표체단백질봉,이지지향량궤방법구건판별모형.결과 FAP여산발성장선류상비,P<0.01적단백질봉유6개,기중질하비위5640、3160、4180화4290적단백질봉재FAP중고표체,질하비위3940화3400적단백질봉재산발성장선류환자중고표체.이질의비분별위5640、3160화4290적단백질봉위기출,연합질하비위3940、13 750화4300적단백질봉소건립적모형판별효과최가,대FAP여산발성장선류적판별준학솔분별위94.7%화93.7%.결론 SELDI-TOF-MS능유효사선FAP여산발성장선류적차이표체단백,지지향량궤방법소건립적질보모형판별효과교호,위진일보연구FAP적분자발병궤제제공료절입점.
Objective To screen out specifically-expressed serum protein markers in familial adenomatous polyposis (FAP) and to establish a serum protein fingerprint diagnostic model for distinguishing FAP from sporadic eolorectal adenomas. Methods Serum samples were collected from 19 FAP cases and 16 sporadic colorectal adenomas with informed consent. Serum protein fingerprint profiles were detected by SELDI-TOF-MS with CM 10 protein chip to screen out FAP adenoma-related serum protein markers, and support vector machine (SVG) technique was used to establish the diagnostic model to distinguish FAP from sporadic eoloreetal adenomas. Results Six differently-expressed protein peaks (P<0.01) were detected. Among them proteins of 5640, 3160, 4180 and 4290 m/z were highly expressed in FAP adenomas, and proteins of 3940 and 3400 m/z were highly expressed in sporadic colorectal adenomas. The accuracy of diagnostic model established with SVG to distinguish FAP adenomas and sporadic colorectal adenomas was 94.7% and 93.7%, respectively. Conclusion SEI,DI-TOF-MS can be effectively used to screen out the differentially expressed serum protein markers in FAP adenomas and sporadic colorectal adenomas, and a diagnostic model build by SVG to distinguish them has been successfully established. Therefore, a useful breakthrough point for research on molecular mechanisms of FAP pathogenesis is provided.