CAJ | 학술논문

目的比较骨髓间充质干细胞(BMSCs)、成骨诱导后的BMSCs以及二者联合经皮移植后体内成骨能力的差异,为骨组织工程选择种子细胞提供依据.方法取第2代BMSCs进行成骨诱导.分别将BMSCs、成骨诱导的BMSCs、BMSCs联合成骨诱导后的BMSCs、生理盐水经皮注入兔桡骨中段骨缺损处.通过组织学、放射学、生物力学方法在不同时相比较骨缺损区修复情况.结果 BMSCs与成骨诱导后的BMSCs联合移植组在12周内骨缺损区新生骨的数量和质量显著优于BMSCs移植组和成骨诱导的BMSCs移植组(F=226.6,P＜0.01),空白组骨缺损主要由纤维结缔组织填充.结论 BMSCs与成骨诱导后的BMSCs联合移植存在协同效应,具有更强的成骨能力,可作为种子细胞应用于骨组织工程.
목적 비교골수간충질간세포(BMSCs)、성골유도후적BMSCs이급이자연합경피이식후체내성골능력적차이,위골조직공정선택충자세포제공의거.방법 취제2대BMSCs진행성골유도.분별장BMSCs、성골유도적BMSCs、BMSCs연합성골유도후적BMSCs、생리염수경피주입토뇨골중단골결손처.통과조직학、방사학、생물역학방법재불동시상비교골결손구수복정황.결과 BMSCs여성골유도후적BMSCs연합이식조재12주내골결손구신생골적수량화질량현저우우BMSCs이식조화성골유도적BMSCs이식조(F=226.6,P＜0.01),공백조골결손주요유섬유결체조직전충.결론 BMSCs여성골유도후적BMSCs연합이식존재협동효응,구유경강적성골능력,가작위충자세포응용우골조직공정.
Objectives To investigate in vivo osteogenic potential in size-critical bone defect after percutaneous autologous grafting of culture-expanded rabbit autologous BMSCs, osteo-induced BMSCs and combination of both. Methods BMSCs were cultured and then induced with osteogenic supplement ( OS) medium. BMSCs with and without OS induction were collected and percutaneously autologously injected respectively into the 15 mm bone defect of 20 experimental rabbit model. The grafts were BMSCs, osteo-induced BMSCs, BMSCs and osteo-induced BMSCs, BMP combined with fibrin sealant, and 0.9% NaCl solution. Osteogenesis at the defect areas were observed by regular radiography, histology and biomechanics. Results the group transplanted with BMSCs + osteo-induced BMSCs aehived complete bone healing with medullary cavity united, which showed the largest quantity of new bone measured by X-ray analysis, and also their maximal load were better than those in other groups. Conclusion The bone-forming ability of rabbit osteo-induced BMSCs combined with BMSCs in bone defect is superior to those of BMSCs and osteo-induced BMSCs.