中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
1期
86-88
,共3页
李晓林%邹小明%李云龙%宋茂力%李刚
李曉林%鄒小明%李雲龍%宋茂力%李剛
리효림%추소명%리운룡%송무력%리강
小肠移植%一氧化氮合成酶%急性排斥反应
小腸移植%一氧化氮閤成酶%急性排斥反應
소장이식%일양화담합성매%급성배척반응
Intestinal transplantation%Nitric oxide synthase%Acute rejection
目的 观察神经型(nNOS)和诱导型一氧化氮合成酶(iNOS)在大鼠小肠移植急性排斥反应(AR)中作用.方法 行大鼠原位小肠移植.实验分为2组.1组:同系移植组(Lewis→Lewis,12例)；2组:同种移植组(DA→Lewis,12例).观察术后生存时间.再灌注30 min、术后1、3、5、7d检测血清一氧化氮(NO)浓度；开腹行麦芽糖吸收实验；切取移植肠管,苏木素-伊红(HE)染色后光镜检查.免疫组织化学法观察移植肠nNOS和iNOS的活性.逆转录-聚合酶链反应(RT-PCR)法检测移植肠nNOS mRNA和iNOS mRNA的表达.结果 A组生存时间＞30 d.B组生存时间为(6.83±0.75)d.再灌注后A组nNOS染色与mRNA表达明显减弱,此后nNOS染色和mRNA表达分别于术后3、7d恢复正常.再灌注后A组iNOS染色与mRNA表达增强,此后逐渐减弱.与A组比较,术后3～7 d,B组nNOS染色减弱,iNOS染色增强,血清NO水平明显升高(P＜0.05),血糖吸收值显著降低(P＜0.01)；术后5、7d,B组nNOS mRNA表达显著下降(P＜0.001),iNOS mRNA表达明显增强(P＜0.01).结论 在AR过程中,nNOS可能调节了iNOS的表达；nNOS的活性和表达与移植肠管的结构和吸收功能密切相关；iNOS的激活是加重组织损伤的重要因素之一.
目的 觀察神經型(nNOS)和誘導型一氧化氮閤成酶(iNOS)在大鼠小腸移植急性排斥反應(AR)中作用.方法 行大鼠原位小腸移植.實驗分為2組.1組:同繫移植組(Lewis→Lewis,12例)；2組:同種移植組(DA→Lewis,12例).觀察術後生存時間.再灌註30 min、術後1、3、5、7d檢測血清一氧化氮(NO)濃度；開腹行麥芽糖吸收實驗；切取移植腸管,囌木素-伊紅(HE)染色後光鏡檢查.免疫組織化學法觀察移植腸nNOS和iNOS的活性.逆轉錄-聚閤酶鏈反應(RT-PCR)法檢測移植腸nNOS mRNA和iNOS mRNA的錶達.結果 A組生存時間＞30 d.B組生存時間為(6.83±0.75)d.再灌註後A組nNOS染色與mRNA錶達明顯減弱,此後nNOS染色和mRNA錶達分彆于術後3、7d恢複正常.再灌註後A組iNOS染色與mRNA錶達增彊,此後逐漸減弱.與A組比較,術後3～7 d,B組nNOS染色減弱,iNOS染色增彊,血清NO水平明顯升高(P＜0.05),血糖吸收值顯著降低(P＜0.01)；術後5、7d,B組nNOS mRNA錶達顯著下降(P＜0.001),iNOS mRNA錶達明顯增彊(P＜0.01).結論 在AR過程中,nNOS可能調節瞭iNOS的錶達；nNOS的活性和錶達與移植腸管的結構和吸收功能密切相關；iNOS的激活是加重組織損傷的重要因素之一.
목적 관찰신경형(nNOS)화유도형일양화담합성매(iNOS)재대서소장이식급성배척반응(AR)중작용.방법 행대서원위소장이식.실험분위2조.1조:동계이식조(Lewis→Lewis,12례)；2조:동충이식조(DA→Lewis,12례).관찰술후생존시간.재관주30 min、술후1、3、5、7d검측혈청일양화담(NO)농도；개복행맥아당흡수실험；절취이식장관,소목소-이홍(HE)염색후광경검사.면역조직화학법관찰이식장nNOS화iNOS적활성.역전록-취합매련반응(RT-PCR)법검측이식장nNOS mRNA화iNOS mRNA적표체.결과 A조생존시간＞30 d.B조생존시간위(6.83±0.75)d.재관주후A조nNOS염색여mRNA표체명현감약,차후nNOS염색화mRNA표체분별우술후3、7d회복정상.재관주후A조iNOS염색여mRNA표체증강,차후축점감약.여A조비교,술후3～7 d,B조nNOS염색감약,iNOS염색증강,혈청NO수평명현승고(P＜0.05),혈당흡수치현저강저(P＜0.01)；술후5、7d,B조nNOS mRNA표체현저하강(P＜0.001),iNOS mRNA표체명현증강(P＜0.01).결론 재AR과정중,nNOS가능조절료iNOS적표체；nNOS적활성화표체여이식장관적결구화흡수공능밀절상관；iNOS적격활시가중조직손상적중요인소지일.
Objective To evaluate the role of neuronal (nNOS) and inducible nitric oxide synthase (iNOS) in acute rejection (AR) of intestinal transplantation in rats.Methods The rat orthotopic intestinal transplantation was performed.Animals were assigned to following 2 groups:isograft group ( A,Lewis→Lewis,n =12) ; allograft group (B,DA→Lewis,n =12).The survival time was observed.At 30 min,postoperative day (POD) 1,3,5 and 7,the serum nitric oxide (NO) levels were measured,the maltose absorption tests were performed,and intestinal specimens were resected for light microscopy after H&E staining.The activity of nNOS and iNOS was examined by immunohistochemistry.The expression levels of nNOS and iNOS mRNA were detected by using reverse transcription-polymerase chain reaction (RT-PCR).Results The survival time in groups A and B was ＞ 30 and (6.83 ±0.75 ) days respectively.In group A,the staining and mRNA expression of nNOS were weakened obviously after reperfusion and returned to normal degree on POD 3 and 7 respectively.The staining and mRNA expression of iNOS were increased after reperfusion and then decreased gradually.As compared with group A,from POD 3 to 7,the nNOS staining was weakened,the iNOS staining was strengthened,the serum NO levels were increased significantly (P ＜ 0.05 ),and the blood glucose absorption levels reduced significantly (P ＜ 0.01 ) in group B.On POD 5 and 7,the nNOS mRNA expression levels were decreased significantly (P ＜ 0.01 ) and the iNOS mRNA expression levels increased significantly (P ＜ 0.001 ) in group B.Conclusion During the AR period,nNOS regulates the expression of iNOS probably.The activity and expression of nNOS have close relations with the graft structure and absorptive function.The activity of iNOS is one of important factors in exacerbating tissue damage.
