CAJ | 학술논문

目的观察以壳聚糖一胶原偶联的生物膜为载体,联合移植雪旺细胞(SCs)与神经干细胞(NSCs)在坐骨神经损伤修复过程中所起的作用,评价该方法的疗效.方法分离纯化SCs和NSCs,传代4代后共培养于壳聚糖一胶原生物膜上.32只Wistar成鼠(体重350～400 g)建立坐骨神经缺损动物模型,随机分为4组:空白组;SCs移植组;NSCs移植组;NSCs联合SCs移植组.14周后观察Cy3荧光素染色、MBP免疫酶染色及苏木素-伊红(HE)染色的结果.结果 BDA示踪显示术后联合移植组和SCs组有较多的神经纤维穿过缺损部位,大鼠右后肢感觉和运动功能恢复较其他组明显,修复神经段神经传导速度比值也大于其他组(P<0.05).SCs组和联合移植组间差异无统计学意义(P>0.05).结论 NSCs和SCs与壳聚糖一胶原生物膜相容性良好,壳聚糖一胶原生物膜介导的SCs联合NSCs桥接周围神经缺损可使部分神经再生.
목적 관찰이각취당일효원우련적생물막위재체,연합이식설왕세포(SCs)여신경간세포(NSCs)재좌골신경손상수복과정중소기적작용,평개해방법적료효.방법 분리순화SCs화NSCs,전대4대후공배양우각취당일효원생물막상.32지Wistar성서(체중350～400 g)건립좌골신경결손동물모형,수궤분위4조:공백조;SCs이식조;NSCs이식조;NSCs연합SCs이식조.14주후관찰Cy3형광소염색、MBP면역매염색급소목소-이홍(HE)염색적결과.결과 BDA시종현시술후연합이식조화SCs조유교다적신경섬유천과결손부위,대서우후지감각화운동공능회복교기타조명현,수복신경단신경전도속도비치야대우기타조(P<0.05).SCs조화연합이식조간차이무통계학의의(P>0.05).결론 NSCs화SCs여각취당일효원생물막상용성량호,각취당일효원생물막개도적SCs연합NSCs교접주위신경결손가사부분신경재생.
Objective To evaluate the effect of bridging the defects of sciatic nerve in Wistar rats by transplantation of Schwann cells (SCs) and neural stem cells (NSCs) embedded to the chitosan-collagen complex film. Methods SCs and NSCs were isolated and purified. These clones were passaged continuously for 4 generations. Then the two types of cells were embedded to the chitosan-collagen complex film. Thirty-two adult Wistar rats, weighing 350-400 g, were divided into 4 groups at random:Blank group, SCs group,NSCs group and SCs + NSCs group. An about 10 mm gap of right sciatic nerve was cut in all rats. The films planted by SCs and/or NSCs or none were transplanted to bridge the sciatic nerve defects. Fourteen weeks later MBP was detected by using cy3 fluorescence staining, HE staining, and immunohistochemistry staining. The velocity of electrical conduction of sciatic nerves was calculated. Results The recovery of sense and motion function in SCs + NSCs group and SCs group were better than the other two groups. SCs + NSCs group and SCs group were superior to the other two groups on neurofibril regeneration and velocity of electrical conduction of sciatic nerve ( P > 0.05 ). But there was no significant difference between SCs group and SCs + NSCs group. Conclusion The two types of cells have a good compatibility with the chitosan-collagen complex film. Transplantation of SCs and NSCs embedded to the chitosan-collagen complex film can guide the regeneration of neurofibrils.