中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2010年
3期
161-165
,共5页
王熠钊%徐江%许涛%方征宇%徐琦%黄晓琳
王熠釗%徐江%許濤%方徵宇%徐琦%黃曉琳
왕습쇠%서강%허도%방정우%서기%황효림
光化学%脊髓损伤%猫
光化學%脊髓損傷%貓
광화학%척수손상%묘
Photochemistry%Spinal cord injury%Cat
目的 观察光化学法诱导猫脊髓损伤后的神经行为学、运动诱发电位及形态学改变,为研究脊髓损伤后神经再生与功能重建建立动物模型.方法 将15只猫根据冷光源照射时间不同随机分为40 min组、60 min组和80 min组.手术暴露脊髓后,用35 mg/kg体重的玫瑰红静脉注射,联合应用强度为3000 klx的冷光源照射T_13脊髓节段.术后21 d内连续观察动物神经行为学改变;于术后第21天检测动物的运动诱发电位,并与术前结果 比较;取材观察动物脊髓组织形态学改变.结果 3组动物在手术后21 d内的神经行为学评分结果 显示,损伤后动物下肢功能神经行为学评分降低,损伤程度与光照时间成正比.60 min组和80 min组动物损伤后运动诱发电位波形消失.3组动物的脊髓都有明显的缺血性坏死改变.结论 光化学法可诱导猫脊髓内产生微血栓,从而使组织发生缺血性坏死.冷光源照射60 min、80 min的动物双下肢运动功能明显受损,与其病理学改变和电生理学改变相符,用这种方法 制作不完全脊髓损伤模型稳定可靠.
目的 觀察光化學法誘導貓脊髓損傷後的神經行為學、運動誘髮電位及形態學改變,為研究脊髓損傷後神經再生與功能重建建立動物模型.方法 將15隻貓根據冷光源照射時間不同隨機分為40 min組、60 min組和80 min組.手術暴露脊髓後,用35 mg/kg體重的玫瑰紅靜脈註射,聯閤應用彊度為3000 klx的冷光源照射T_13脊髓節段.術後21 d內連續觀察動物神經行為學改變;于術後第21天檢測動物的運動誘髮電位,併與術前結果 比較;取材觀察動物脊髓組織形態學改變.結果 3組動物在手術後21 d內的神經行為學評分結果 顯示,損傷後動物下肢功能神經行為學評分降低,損傷程度與光照時間成正比.60 min組和80 min組動物損傷後運動誘髮電位波形消失.3組動物的脊髓都有明顯的缺血性壞死改變.結論 光化學法可誘導貓脊髓內產生微血栓,從而使組織髮生缺血性壞死.冷光源照射60 min、80 min的動物雙下肢運動功能明顯受損,與其病理學改變和電生理學改變相符,用這種方法 製作不完全脊髓損傷模型穩定可靠.
목적 관찰광화학법유도묘척수손상후적신경행위학、운동유발전위급형태학개변,위연구척수손상후신경재생여공능중건건립동물모형.방법 장15지묘근거랭광원조사시간불동수궤분위40 min조、60 min조화80 min조.수술폭로척수후,용35 mg/kg체중적매괴홍정맥주사,연합응용강도위3000 klx적랭광원조사T_13척수절단.술후21 d내련속관찰동물신경행위학개변;우술후제21천검측동물적운동유발전위,병여술전결과 비교;취재관찰동물척수조직형태학개변.결과 3조동물재수술후21 d내적신경행위학평분결과 현시,손상후동물하지공능신경행위학평분강저,손상정도여광조시간성정비.60 min조화80 min조동물손상후운동유발전위파형소실.3조동물적척수도유명현적결혈성배사개변.결론 광화학법가유도묘척수내산생미혈전,종이사조직발생결혈성배사.랭광원조사60 min、80 min적동물쌍하지운동공능명현수손,여기병이학개변화전생이학개변상부,용저충방법 제작불완전척수손상모형은정가고.
Objective To explore the potential of creating a cat model of spinal cord infarction initiated by a photochemical reaction in terms of the neuroethology, motor evoked potential, and morphological outcomes. Meth-ods Fifteen cats were divided into three groups at random. T_13 in the spinal cord was photochemically irradiated for 40 min, 60 min or 80 min in different groups. A photochemically-induced infarction was produced by intravenous in-jection of rose Bengal (35 mg/kg) combined with immediate cold light irradiation (3000 klx) of the spinal cord.Neuroethology changes were observed every day after the surgery far 21 days, and morphological changes were exam-ined at day 21, before which the motor evoked potential was examined and compared with measurements taken before injury. Results The spinal cord infarctions induced by intravenous injection of rose Bengal plus cold light irradia-tion for 40 min were stable by day 8, while the other two groups were stable by day 12. The size of the infarction area in the spinal cord was stable, while the neuroethology, electrophysiological and histopathological changes in the cats were significant. Conclusions All of the cats demonstrated decreased functional mobility after photochemically in-duced thrombosis of the spinal cord, with corresponding pathomorphological and electrophysiologic changes. The model of infarction was stable and reliable.