中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2011年
10期
742-745
,共4页
张杰%陆洪英%金成文%成敏
張傑%陸洪英%金成文%成敏
장걸%륙홍영%금성문%성민
恒磁场%内皮细胞%一氧化氮%内皮素-1%6-酮-前列环素
恆磁場%內皮細胞%一氧化氮%內皮素-1%6-酮-前列環素
항자장%내피세포%일양화담%내피소-1%6-동-전렬배소
Static magnetic fields%Endothelial cells%Nitric oxide%Endothelin-1%6-keto-prostacyclin 1 α
目的 观察不同强度的恒磁场作用不同时间对培养的人脐静脉内皮细胞(HUVECs)增殖、凋亡及分泌功能的影响.方法 将HUVECs分别暴露于场强为5、22、86或135 mT的恒磁场中,磁场作用时间分别为8、12或24 h.以流式细胞技术分析恒磁场对HUVECs增殖及凋亡的影响；比色法测定细胞培养液中一氧化氮(NO)含量的变化；放射免疫法测定细胞培养液中的内皮素-1( ET-1)及6-酮-前列环素F1α(6-keto-PGF1α)的含量.结果 ①低强度磁场(5 mT)短时间(8 h)作用于HUVECs可促使其增殖,但高强度磁场( 135 mT)或作用时间过长(12 h或24 h)则会抑制HUVECs的增殖；②磁场对HUVECs凋亡无影响；③低强度磁场(5 mT)短时间(8 h)作用可促使HUVECs合成释放NO和6-keto-PGF1α,但高强度磁场( 135 mT)或作用时间过长(12 h或24 h)则会抑制HUVECs合成分泌NO和6-keto-PGF1α；④细胞暴露于磁场8h时,5 mT组和22 mT组与对照组比较,HUVECs合成释放ET-1量无明显变化(P＞0.05),但86 mT组和135 mT组的细胞ET-1分泌量高于对照组(P＜0.01).当磁场作用时间分别为12和24 h时,5 mT组与对应时间点对照组比较,细胞ET-1合成释放量仍无变化(P＞0.05),22 mT组、86 mT组、135 mT组则明显低于对照组(P＜0.01).结论 低强度磁场、短时间作用可促进HUVECs的增殖及舒血管物质的合成分泌,而高强度磁场或磁场作用时间过长则抑制HUVECs增殖,增加缩血管物质的合成.
目的 觀察不同彊度的恆磁場作用不同時間對培養的人臍靜脈內皮細胞(HUVECs)增殖、凋亡及分泌功能的影響.方法 將HUVECs分彆暴露于場彊為5、22、86或135 mT的恆磁場中,磁場作用時間分彆為8、12或24 h.以流式細胞技術分析恆磁場對HUVECs增殖及凋亡的影響；比色法測定細胞培養液中一氧化氮(NO)含量的變化；放射免疫法測定細胞培養液中的內皮素-1( ET-1)及6-酮-前列環素F1α(6-keto-PGF1α)的含量.結果 ①低彊度磁場(5 mT)短時間(8 h)作用于HUVECs可促使其增殖,但高彊度磁場( 135 mT)或作用時間過長(12 h或24 h)則會抑製HUVECs的增殖；②磁場對HUVECs凋亡無影響；③低彊度磁場(5 mT)短時間(8 h)作用可促使HUVECs閤成釋放NO和6-keto-PGF1α,但高彊度磁場( 135 mT)或作用時間過長(12 h或24 h)則會抑製HUVECs閤成分泌NO和6-keto-PGF1α；④細胞暴露于磁場8h時,5 mT組和22 mT組與對照組比較,HUVECs閤成釋放ET-1量無明顯變化(P＞0.05),但86 mT組和135 mT組的細胞ET-1分泌量高于對照組(P＜0.01).噹磁場作用時間分彆為12和24 h時,5 mT組與對應時間點對照組比較,細胞ET-1閤成釋放量仍無變化(P＞0.05),22 mT組、86 mT組、135 mT組則明顯低于對照組(P＜0.01).結論 低彊度磁場、短時間作用可促進HUVECs的增殖及舒血管物質的閤成分泌,而高彊度磁場或磁場作用時間過長則抑製HUVECs增殖,增加縮血管物質的閤成.
목적 관찰불동강도적항자장작용불동시간대배양적인제정맥내피세포(HUVECs)증식、조망급분비공능적영향.방법 장HUVECs분별폭로우장강위5、22、86혹135 mT적항자장중,자장작용시간분별위8、12혹24 h.이류식세포기술분석항자장대HUVECs증식급조망적영향；비색법측정세포배양액중일양화담(NO)함량적변화；방사면역법측정세포배양액중적내피소-1( ET-1)급6-동-전렬배소F1α(6-keto-PGF1α)적함량.결과 ①저강도자장(5 mT)단시간(8 h)작용우HUVECs가촉사기증식,단고강도자장( 135 mT)혹작용시간과장(12 h혹24 h)칙회억제HUVECs적증식；②자장대HUVECs조망무영향；③저강도자장(5 mT)단시간(8 h)작용가촉사HUVECs합성석방NO화6-keto-PGF1α,단고강도자장( 135 mT)혹작용시간과장(12 h혹24 h)칙회억제HUVECs합성분비NO화6-keto-PGF1α；④세포폭로우자장8h시,5 mT조화22 mT조여대조조비교,HUVECs합성석방ET-1량무명현변화(P＞0.05),단86 mT조화135 mT조적세포ET-1분비량고우대조조(P＜0.01).당자장작용시간분별위12화24 h시,5 mT조여대응시간점대조조비교,세포ET-1합성석방량잉무변화(P＞0.05),22 mT조、86 mT조、135 mT조칙명현저우대조조(P＜0.01).결론 저강도자장、단시간작용가촉진HUVECs적증식급서혈관물질적합성분비,이고강도자장혹자장작용시간과장칙억제HUVECs증식,증가축혈관물질적합성.
Objective To evaluate the effects of static magnetic fields (SMFs) of different intensity and exposure duration on the proliferation and apoptosis of human umbilical cord endothelial cells (HUVECs),and their release of nitric oxide (NO),6-keto-prostacyclin 1α (6-keto-PGF1α) and endothelin (ET-1).Methods Cultured HUVECs were exposed to a SMF at 5,22,86 or 135 mT for 8,12 or 24 hours.Their proliferation and apoptosis were monitored by flow cytometry (FCM).The medium was collected to test its NO content by optical density.ET-1 and 6-keto-PGF1α were measured by radioimmunization.Results ( 1 ) The proliferation of HUVECs increased when the cells were exposed to a SMF at 5 mT for 8 h,but a SMF at 135 mT for 12 h or 24 h inhibited the proliferation of HUVECs.(2)An SMF had no effect on apoptosis of HUVECs.(3)An SMF at 5 mT for 8 h increased the release of NO and 6-keto-PGF1 a,but the release of NO and 6-keto-PGF1 a decreased when the SMF intensity was 135 mT or the cells were exposed to an SMF for 12 h or 24 h.(4) An SMF at 5 mT or 22 mT for 8 h did not effect the release of ET-1.An SMF at 86 mT or 135 mT increased the release of ET-1.Compared with a control group,an SMF at 5 mT for 12 or 24 h did not affect the release of ET1,but at 22,80 or 135 mT,the release of ET-1 decreased significantly.Conclusions Exposure to a low intensity SMF for a short duration could improve the proliferation of HUVECs and increase the release of vasoactive factors,but if HUVECs are exposed to a strong SMF or exposed for a long duration,the proliferation and the release of vasoactive factors is decreased.
