CAJ | 학술논문

目的探索建立转突变型淀粉样蛋白前体(APP)双荧光融合基因(CFP-54 bp-YFP-C99)转基因小鼠模型的可行性和基因整合效率.方法采用显微注射法,将构建的突变型APP双荧光融合基因注入昆明小白鼠的受精卵,获取子代鼠,通过聚合酶链反应(PCR)方法初步鉴定基因的整合情况,以获得阳性鼠.结果注射昆明小白鼠受精卵共2202枚,移植1806枚.受体鼠56只,怀孕鼠13只,共生产52只子代鼠,其中存活32只.PCR初步检查整合有突变型APP双荧光融合基因的阳性鼠2只,均为活鼠.移植鼠的总怀孕率为23.2%(13/56),突变型APP双荧光融合基因的整合效率为3.9%(2/52).结论显微注射法建立转突变型APP双荧光融合基因的阿尔茨海默病小鼠模型是可行的,但转基因效率较低.
목적 탐색건립전돌변형정분양단백전체(APP)쌍형광융합기인(CFP-54 bp-YFP-C99)전기인소서모형적가행성화기인정합효솔.방법 채용현미주사법,장구건적돌변형APP쌍형광융합기인주입곤명소백서적수정란,획취자대서,통과취합매련반응(PCR)방법초보감정기인적정합정황,이획득양성서.결과 주사곤명소백서수정란공2202매,이식1806매.수체서56지,부잉서13지,공생산52지자대서,기중존활32지.PCR초보검사정합유돌변형APP쌍형광융합기인적양성서2지,균위활서.이식서적총부잉솔위23.2%(13/56),돌변형APP쌍형광융합기인적정합효솔위3.9%(2/52).결론 현미주사법건립전돌변형APP쌍형광융합기인적아이자해묵병소서모형시가행적,단전기인효솔교저.
Objective To develop transgenic mice harboring the fusion gene of mutant amyloid precursor protein and two types of fluorescent protein for the future study on Alzheimer's disease.Methods The fusion gene CFP-54 bp-YFP-C99 was introduced into mice by mieroinjection.The presence of CFP-54 bp-YFP-C99 was confirmed by PCR in the founders.Results CFP-54 bp-YFP-C99 gene was injected into pronucleus of 2202 zygotes and 1806 injected eggs were implanted into 56 foster mothers, 13 of which were pregnant.There were 13 foster mothers who borne 52 offspring and 32 of them survived.Recipient mouse pregnancy rate was 23.2% (13/56) and the integration rate was 3.9% (2/52).Conclusion CFP-54 bp-YFP-C99 transgenic mice is obtained, but the transgenic efficiency is low.