CAJ | 학술논문

目的探讨胎兔皮肤伤口无瘢痕愈合机理。方法取胎兔、孕兔、成年兔切口及其周围组织，用阿利新蓝比色法，醋酸纤维素膜电泳分离法测定糖胺多糖(GAG)和透明质酸(HA)含量。结果①胎兔正常皮肤GAG及HA的含量均明显高于孕兔及成年兔(P＜0.01)。②手术区，胎兔、孕兔、成年兔在术后3d时GAG及HA均升高(P＜0.05)。但术后第7d，胎兔GAG含量仍维持在高水平，而孕兔及成年兔其含量下降到接近正常水平。结论高浓度的HA在胎兔伤口间质的持续存在对胎兔伤口无瘢痕愈合起很重要的作用
목적탐토태토피부상구무반흔유합궤리。방법취태토、잉토、성년토절구급기주위조직，용아리신람비색법，작산섬유소막전영분리법측정당알다당(GAG)화투명질산(HA)함량。결과①태토정상피부GAG급HA적함량균명현고우잉토급성년토(P＜0.01)。②수술구，태토、잉토、성년토재술후3d시GAG급HA균승고(P＜0.05)。단술후제7d，태토GAG함량잉유지재고수평，이잉토급성년토기함량하강도접근정상수평。결론고농도적HA재태토상구간질적지속존재대태토상구무반흔유합기흔중요적작용
Objective To investigate the mechanism of the fetal scare-less wound healing(FSWH)in rabbits. Methods Glycosaminoglycan(GAG) and Hyaluronic Acid(HA) of the wounded tissue and normal skin from the fetal, maternal and adult rabbits were measured using the techniques of chromatometry and electrophoresis. Results (1) In the normal skin, the content of GAG and HA of the fetal is about 2.5 times that of the adult and maternal rabbits (P＜0.01). (2) Three days after wounding, the content of GAG of the wounded tissue increased significantly (P＜0.01). At 7 days, the contents of both GAG and HA maintained at a high level(P＜0.01) in the fetus while the levels decreased to normal in the maternal and adult animals. Conclusion Our results showed that the continual existing of a HA-rich matrix played an important role in fetal scar less wound healing in rabbits.