CAJ | 학술논문

目的比较50%胎牛血清,油酸分别建立HL-7702细胞脂肪肝的离体细胞模型的优缺点.方法采用50%胎牛血清、油酸分别建立HL-7702细胞脂肪肝的离体细胞模型,并用四甲基偶氮唑盐法检测细胞活性,油红O染色观察细胞内脂滴数量、甘油-3-磷酸氧化酶法检测细胞内甘油三酯含量,比较两种方法的优缺点.两组计量数据比较用t检验,多个样本均数比较采用方差分析.结果 0.5mmol/ml油酸组和50%胎牛血清组都能建立较好的肝细胞脂肪变性模型,其中0.5 mmol/ml油酸诱导的细胞脂变更为明显,细胞内甘油三酯含量为(893.2±89.1)μg/mg,50%胎牛血清组细胞内甘油三酯含量为(411.6±126.6)μg/mg,两组比较,F=72.288,P＜0.01,差异有统计意义.结论 0.5 mmol/ml油酸诱导的肝细胞脂肪变性模型具有实用性和可重复性.该模型为非酒精性脂肪性肝病的研究提供了一条有效、简便、可行的新途径.
목적 비교50%태우혈청,유산분별건립HL-7702세포지방간적리체세포모형적우결점.방법 채용50%태우혈청、유산분별건립HL-7702세포지방간적리체세포모형,병용사갑기우담서염법검측세포활성,유홍O염색관찰세포내지적수량、감유-3-린산양화매법검측세포내감유삼지함량,비교량충방법적우결점.량조계량수거비교용t검험,다개양본균수비교채용방차분석.결과 0.5mmol/ml유산조화50%태우혈청조도능건립교호적간세포지방변성모형,기중0.5 mmol/ml유산유도적세포지변경위명현,세포내감유삼지함량위(893.2±89.1)μg/mg,50%태우혈청조세포내감유삼지함량위(411.6±126.6)μg/mg,량조비교,F=72.288,P＜0.01,차이유통계의의.결론 0.5 mmol/ml유산유도적간세포지방변성모형구유실용성화가중복성.해모형위비주정성지방성간병적연구제공료일조유효、간편、가행적신도경.
Objective To compare two models of nonalcoholic hepatocellular stoatosis.Methods HL-7702 cells were incubated with a mixture of of unsaturated oleate acid or 50%fetal bovine serum to induce fat-overloading.Significant fat accumulation was documented by Oil Red O staining,and intracellular triglyceride levels was detected by triglyceride enzymatic assay.Results The results showed that both 0.5 mmol/ml oleate acid and 50%FBS were able to induce nonalcohoHc hepatocellular steatosis.Conclusion A nonalcoholic hepatocellular steatosis was induced by 0.5 mmol/ml oleate acid.