国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2012年
4期
193-197
,共5页
唐剑频%蒋丰慧%高玉振%石美森%许传超%陈锐
唐劍頻%蔣豐慧%高玉振%石美森%許傳超%陳銳
당검빈%장봉혜%고옥진%석미삼%허전초%진예
短串联重复序列(STR)%X染色体%单倍型%连锁不平衡
短串聯重複序列(STR)%X染色體%單倍型%連鎖不平衡
단천련중복서렬(STR)%X염색체%단배형%련쇄불평형
Short tandem repeat (STR)%Chromosome X%Haplotype%Linkage disequilibrium
目的 调查苏州汉族群体中5个X-STR的等位基因和单倍型分布,以及分析这些基因座间的连锁不平衡关系.方法 应用Chelex法提取样本DNA,PCR扩增5个X-STR,应用自动测序仪对PCR产物进行分型.结果 苏州汉族群体中,DXS7423、DXS10147、DXS10146、DXS10134、DXS8377分别观察到5、6、15、21、15个等位基因；男女性样本间,DXS7423、DXS10147、DXS10134的等位基因频率差异无统计学意义,P值分别为0.161、0.756、0.179;DXS10146、DXS8377等位基因频率差异具有统计学意义,P值分别为0.008和0.005.5个X-STR的观察杂合度在0.505～0.845间,DPf值在0.684～0.977间,DPm值在0.487 ～0.902间,MCEtrio值在0.469～0.894间,MCEduo值在0.266～0.860间.男性样本中,观察到111种单倍型,单倍型变异度为0.9916.结论 苏州汉族群体中,5个X-STR中DXS10146、DXS10134、DXS8377多态性较好；5个X- STR构成的单倍型鉴别能力较高.
目的 調查囌州漢族群體中5箇X-STR的等位基因和單倍型分佈,以及分析這些基因座間的連鎖不平衡關繫.方法 應用Chelex法提取樣本DNA,PCR擴增5箇X-STR,應用自動測序儀對PCR產物進行分型.結果 囌州漢族群體中,DXS7423、DXS10147、DXS10146、DXS10134、DXS8377分彆觀察到5、6、15、21、15箇等位基因；男女性樣本間,DXS7423、DXS10147、DXS10134的等位基因頻率差異無統計學意義,P值分彆為0.161、0.756、0.179;DXS10146、DXS8377等位基因頻率差異具有統計學意義,P值分彆為0.008和0.005.5箇X-STR的觀察雜閤度在0.505～0.845間,DPf值在0.684～0.977間,DPm值在0.487 ～0.902間,MCEtrio值在0.469～0.894間,MCEduo值在0.266～0.860間.男性樣本中,觀察到111種單倍型,單倍型變異度為0.9916.結論 囌州漢族群體中,5箇X-STR中DXS10146、DXS10134、DXS8377多態性較好；5箇X- STR構成的單倍型鑒彆能力較高.
목적 조사소주한족군체중5개X-STR적등위기인화단배형분포,이급분석저사기인좌간적련쇄불평형관계.방법 응용Chelex법제취양본DNA,PCR확증5개X-STR,응용자동측서의대PCR산물진행분형.결과 소주한족군체중,DXS7423、DXS10147、DXS10146、DXS10134、DXS8377분별관찰도5、6、15、21、15개등위기인；남녀성양본간,DXS7423、DXS10147、DXS10134적등위기인빈솔차이무통계학의의,P치분별위0.161、0.756、0.179;DXS10146、DXS8377등위기인빈솔차이구유통계학의의,P치분별위0.008화0.005.5개X-STR적관찰잡합도재0.505～0.845간,DPf치재0.684～0.977간,DPm치재0.487 ～0.902간,MCEtrio치재0.469～0.894간,MCEduo치재0.266～0.860간.남성양본중,관찰도111충단배형,단배형변이도위0.9916.결론 소주한족군체중,5개X-STR중DXS10146、DXS10134、DXS8377다태성교호；5개X- STR구성적단배형감별능력교고.
Objective To investigate the frequencies of allele and haplotype of 5 X-STR in Suzhou Han population,and to analyze the linkage disequilibrium among 5 X-STR loci.Methods Genomic DNA of samples was extracted using Chelex method.All loci were amplified using PCR,and were genotyped using the automatic sequencer. Results A total of 5,6,15,21 and 15 alleles at DXS7423,DXS10147,DXS10146,DXS10134 and DXS8377 were observed in Suzhou Han population,respectively. No significant difference of allelic frequencies between females and males was found at DXS7423(P=0.161),DXS10147(P=0.756) and DXS10134(P=0.179).Significant differences of allelic frequencies between females and males were found at DXS10146 and DXS8377 (P=0.008 and 0.005 ).The values of observed heterozygosities range from 0.505 to 0.845 in Suzhou Han population,DPf values vary between 0.684 and 0.977,DPm values vary between 0.487 and 0.902,MCEtrio values range from 0.469 to 0.894,and MCEduo values range from 0.266 to 0.860.A total of 111 haplotypes were observed in males of Suzhou Han population,and haplotype diversity was 0.9916.Conclusion The DXS10146,DXS10134 and DXS8377 loci are more polymorphic than other loci in Suzhou Hanpopulation.The haplotypes composed of 5 loci show good identification efficiency.
