CAJ | 학술논문

1982年10～11月在上海暴发流行期收集的45株鼠伤寒沙门氏菌流行株中,对氨苄青霉素、氯霉素、四环素、庆大霉素、卡那霉素、链霉素、氧哌嗪青霉素和复方SMz-TMP的耐药者占73.3%,对5种或5种以上抗菌药物耐物药者占93.7%;而同期从带菌者中分离的9株鼠伤寒沙门氏菌非流行株中仅工株为多重耐药株.接合转移和转化试验证明,细菌的多重耐药性乃耐药(R)质粒(抗性或耐药质粒)所介导,25%在五质粒可将其耐药性转移给大肠杆菌KRif/12,转移频率为10-4～10-6.琼脂糖凝胶电泳显示:流行株的质粒谱相似+均有5～6个质粒带,其中大质粒与多重耐药性有关;而非流行株仅有一个质粒带.
1982년10～11월재상해폭발류행기수집적45주서상한사문씨균류행주중,대안변청매소、록매소、사배소、경대매소、잡나매소、련매소、양고진청매소화복방SMz-TMP적내약자점73.3%,대5충혹5충이상항균약물내물약자점93.7%;이동기종대균자중분리적9주서상한사문씨균비류행주중부공주위다중내약주.접합전이화전화시험증명,세균적다중내약성내내약(R)질립(항성혹내약질립)소개도,25%재오질립가장기내약성전이급대장간균KRif/12,전이빈솔위10-4～10-6.경지당응효전영현시:류행주적질립보상사+균유5～6개질립대,기중대질립여다중내약성유관;이비류행주부유일개질립대.
From Ooc 10 Nov 1982,45 epidemie strains of S.typhimurium were collected from several outbreaks in Shanghai. 73.8% of them were resistant to ampieillin, chlormaphenicol,tetracycline,gentamiein,kanamycin,streptomyein,piperacillin,and SMZ Co,and 93.2% were resistant to 5 anttmicrobial agents at least.However,only one out of 9 strains,isolated from S,typhimurium carriers,was multiresisfant.By conjugation and transformation,the multiresistance was shown to be plasmid-mediated.25% of R plasmids were transferable to E.coli K12Rlf with a frequency of-4 to 10-6.Electrophoresis in agaross gel of plasmid preparationg showed that the plastaid pattern of epidemic strains were similar,each of them had 5～6 plasmid bands,the larger plasmids were the genes responsible for multiresistance.In contrast,there was only one plasmid band in the non-epidemic strain.