CAJ | 학술논문

背景金纳米颗粒对肿瘤细胞具有杀伤效应.目的观察金纳米链对人喉癌Hep-2 细胞增殖的影响.方法首先运用葡萄糖体系合成法制备金纳米链溶胶,然后MTT 法检测不同终浓度(10%,25%,50%,75%,95%)金纳米链溶胶对人喉癌Hep-2 细胞增殖的影响,并通过电镜观察金纳米链进出Hep-2 细胞的过程.结果与结论金纳米链在75%和95%高浓度时对Hep-2 细胞增殖有一定的抑制,但并没有随着浓度的增加而加重,均属于一级范围,表明金纳米链对人喉癌Hep-2 细胞无毒性.金纳米链在与Hep-2 细胞共培养8 h 后即能以胞吞的方式进入细胞,48 h 后大部分出胞,能够自由进出细胞.
배경 금납미과립대종류세포구유살상효응.목적 관찰금납미련대인후암Hep-2 세포증식적영향.방법 수선운용포도당체계합성법제비금납미련용효,연후MTT 법검측불동종농도(10%,25%,50%,75%,95%)금납미련용효대인후암Hep-2 세포증식적영향,병통과전경관찰금납미련진출Hep-2 세포적과정.결과 여결론 금납미련재75%화95%고농도시대Hep-2 세포증식유일정적억제,단병몰유수착농도적증가이가중,균속우일급범위,표명금납미련대인후암Hep-2 세포무독성.금납미련재여Hep-2 세포공배양8 h 후즉능이포탄적방식진입세포,48 h 후대부분출포,능구자유진출세포.
BACKGROUND: The gold nanoparticles have a killing effect on tumor cells.OBJECTIVE: To investigate the influence of gold nanochain on Hep-2 cells proliferation of human laryngeal carcinoma.METHODS: The gold nanochain was prepared by a glucose synthesis method and added into the culture cells with different concentrations (10%, 25%, 50%, 75%, 95%) to test the influence on proliferation of in vitro cultured Hep-2 cells. The endocytosis and exocytosis of transmembrane when gold nanochain attached to Hep-2 cells were observed by electron microscopy.RESULTS AND CONCLUSION: The gold chain at high concentrations (75%, 95%) exhibited inhibitory effects on the proliferation of Hep-2 cells, but the influence was not increased with increasing concentration, belonging to a range of non-toxic. Gold nanchain can enter into Hep-2 cells after 8 hours of co-culture and leave cells after 48 hours, indicating gold nanoparticles chain can enter and leave Hep-2 freely.