CAJ | 학술논문

[目的]研究影响拟南芥早期胚胎发育分裂模式的基因。[方法]从拟南芥T-DNA插入的突变体库中分离到2个T-DNA插入突变体,通过表型观察胚胎发育情况。[结果]PCR-WALKING表明T-DNA插入位点分别位于基因At4g20360的5＇非编码区和启动子区,基因编码的GTPase RABE1b蛋白为Rab蛋白家族的成员,将这2个突变体分别命名为Atrabe1 b-1和Atrabe1b-2。透明分析发现此突变体在胚胎发育早期球形胚时期分裂模式出现异常,RT-PCR分析发现此基因在拟南芥中以组成型表达。[结论]基因At4g20360影响拟南芥早期胚胎发育的分裂模式,推测其编码的蛋白GTPaseRABE1b可能对控制拟南芥胚胎发育过程中的细胞分裂起重要作用。
[목적]연구영향의남개조기배태발육분렬모식적기인。[방법]종의남개T-DNA삽입적돌변체고중분리도2개T-DNA삽입돌변체,통과표형관찰배태발육정황。[결과]PCR-WALKING표명T-DNA삽입위점분별위우기인At4g20360적5＇비편마구화계동자구,기인편마적GTPase RABE1b단백위Rab단백가족적성원,장저2개돌변체분별명명위Atrabe1 b-1화Atrabe1b-2。투명분석발현차돌변체재배태발육조기구형배시기분렬모식출현이상,RT-PCR분석발현차기인재의남개중이조성형표체。[결론]기인At4g20360영향의남개조기배태발육적분렬모식,추측기편마적단백GTPaseRABE1b가능대공제의남개배태발육과정중적세포분렬기중요작용。
[Objective] This study aimed to study the genes affecting division mode in the early embryonic development of Arabidopsis thaliana.[Method] Two T-DNA insertion mutants were isolated from the mutant library of A.thaliana inserted by T-DNA,and the embryonic development was reflected by phenotypes.[Result] Genomic PCR-WALKING analyses demonstrated that T-DNA insertion sites were in 5＇ non-coding region and promoter region of gene At4g20360 which encodes Rab GTPase RABE1b,and the two mutants were named as Atrabe1b-1 and Atrabe1b-2,respectively.Microscopic analysis on mutants revealed that there was an abnormal cell division pattern in early globular stage of embryonic development.In addition,RT-PCR analysis showed that gene At4g20360 was constitutively expressed in A.thaliana.[Conclusion] Gene At4g20360 affected the division pattern of early embryonic development in A.thaliana,and encoded protein GTPase RABE1b which may play an important role in cell division during the embryonic development of A.thaliana.