延安大学学报:自然科学版
延安大學學報:自然科學版
연안대학학보:자연과학판
Journal of Yan'an University(Natural Science Edition)
2011年
4期
87-89
,共3页
冯晓东%周海华%常海飞%黑淑梅
馮曉東%週海華%常海飛%黑淑梅
풍효동%주해화%상해비%흑숙매
马齿苋%总黄酮%正交试验%超声波细胞破碎法
馬齒莧%總黃酮%正交試驗%超聲波細胞破碎法
마치현%총황동%정교시험%초성파세포파쇄법
Portulaca oleracea L.%total flavonoids%orthogonal experiment%Ultrasionic cell-break method
采用超声波细胞破碎法和索氏提取法提取马齿苋中的总黄酮,用比色法测定总黄酮含量,通过正交试验确定超声波细胞破碎法的最佳提取条件。结果表明,超声波细胞破碎法提取的影响因素顺序为:料液比〉乙醇浓度〉提取时间;用超声波细胞破碎法提取马齿苋中总黄酮的最佳条件为:料液比为1 g∶20 mL、乙醇体积分数为90%、提取时间40 min。测得马齿苋中总黄酮含量为5.63%。
採用超聲波細胞破碎法和索氏提取法提取馬齒莧中的總黃酮,用比色法測定總黃酮含量,通過正交試驗確定超聲波細胞破碎法的最佳提取條件。結果錶明,超聲波細胞破碎法提取的影響因素順序為:料液比〉乙醇濃度〉提取時間;用超聲波細胞破碎法提取馬齒莧中總黃酮的最佳條件為:料液比為1 g∶20 mL、乙醇體積分數為90%、提取時間40 min。測得馬齒莧中總黃酮含量為5.63%。
채용초성파세포파쇄법화색씨제취법제취마치현중적총황동,용비색법측정총황동함량,통과정교시험학정초성파세포파쇄법적최가제취조건。결과표명,초성파세포파쇄법제취적영향인소순서위:료액비〉을순농도〉제취시간;용초성파세포파쇄법제취마치현중총황동적최가조건위:료액비위1 g∶20 mL、을순체적분수위90%、제취시간40 min。측득마치현중총황동함량위5.63%。
Through ultrasionic cell-break method and Soxhlet extractions to extract the flavonoids in Portulaca oleracea L.and determinate its contents by UV spectrophotometry,the best condition of the extraction was selected by using orthogonal experiment.The results showed that factors influencing the extraction were in the order as follows:solid-liquid ratioethanol concentrationextractive time;the optimal extracting conditions extracted the flavonoids in Portulaca oleracea L.by the Ultrasionic cell-break method and Soxhlet extractions is:solid-liquid ratio 1:50(g/mL),ethanol concentration 90%and extractive time 40min.The contents of flavonoids from the Portulaca oleracea L.in Yan'an were 5.63%.