CAJ | 학술논문

从延边地区黑熊肠道中分离并鉴定出3株乳酸菌，其中，嗜酸乳杆菌2株，发酵乳杆菌1株．根据其活性及胆盐耐受性选择2株嗜酸乳杆菌（L-1，L-3）进行耐酸试验、抑菌试验及毒性试验．结果表明，这2株嗜酸乳杆菌在胆盐含量0．3％，pH值为2．0时仍然生长，其代谢产物对常见致病菌有较好抑制作用，对小白鼠的毒性试验无毒性．这表明该菌株完全能适应动物胃肠道环境，作为微生态制剂用菌种具有较好的潜力．
종연변지구흑웅장도중분리병감정출3주유산균，기중，기산유간균2주，발효유간균1주．근거기활성급담염내수성선택2주기산유간균（L-1，L-3）진행내산시험、억균시험급독성시험．결과표명，저2주기산유간균재담염함량0．3％，pH치위2．0시잉연생장，기대사산물대상견치병균유교호억제작용，대소백서적독성시험무독성．저표명해균주완전능괄응동물위장도배경，작위미생태제제용균충구유교호적잠력．
Three strains of the lactic acid bacteria were isolated and identified from the intestinal tracts of Yanbian Black bears, in which two strains are lactobacillus acidophilus, and one strain is lactobacillus fermentative. Two strains of lactobacillus acidophilus L-1 and L-3 were selected according to their tests such as their activity and tolerance to bile salts, acid tolerance, bacteriostasis and toxicity were further tested. The results showed that the two strains of lactobacillus acidophilus can still be alive in the bile salt solution which concentration is 0.3% and pH value is 2.0, the metabolic products of which have good inhibitory effect to pathogenic bacteria, and have no toxicity to white mice. Indicates it can completely adapt to animals＇ intestinal environment and has a good potential for microbial preparation.