沈阳化工大学学报
瀋暘化工大學學報
침양화공대학학보
JOURNAL OF SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
2011年
2期
117-120,143
,共5页
低温碱性蛋白酶%枯草芽孢杆菌%选育%优化
低溫堿性蛋白酶%枯草芽孢桿菌%選育%優化
저온감성단백매%고초아포간균%선육%우화
low-temperature alkaline protease%Bacillus subtilis%breeding%optimization
利用实验室保藏的枯草芽孢杆菌为出发菌株,采用紫外诱变进行选育,得到1株低温碱性蛋白酶的高产菌株,编号为B.Sub3.采用摇瓶液体发酵方式,考察碳源、氮源等营养条件及温度、发酵时间等发酵条件对发酵的影响,结果表明以上因素对低温碱性蛋白酶的生产均有影响.在单因素基础上设计4因素3水平的正交实验,确定B.Sub3的最佳发酵条件为：葡萄糖5 g/L,蛋白胨5 g/L,酵母粉3 g/L,pH=8.0,接种比例为5%,50 mL三角瓶装液量为20 mL,32℃发酵28 h,发酵液的低温蛋白酶活力为2 231 U/mL.
利用實驗室保藏的枯草芽孢桿菌為齣髮菌株,採用紫外誘變進行選育,得到1株低溫堿性蛋白酶的高產菌株,編號為B.Sub3.採用搖瓶液體髮酵方式,攷察碳源、氮源等營養條件及溫度、髮酵時間等髮酵條件對髮酵的影響,結果錶明以上因素對低溫堿性蛋白酶的生產均有影響.在單因素基礎上設計4因素3水平的正交實驗,確定B.Sub3的最佳髮酵條件為：葡萄糖5 g/L,蛋白胨5 g/L,酵母粉3 g/L,pH=8.0,接種比例為5%,50 mL三角瓶裝液量為20 mL,32℃髮酵28 h,髮酵液的低溫蛋白酶活力為2 231 U/mL.
이용실험실보장적고초아포간균위출발균주,채용자외유변진행선육,득도1주저온감성단백매적고산균주,편호위B.Sub3.채용요병액체발효방식,고찰탄원、담원등영양조건급온도、발효시간등발효조건대발효적영향,결과표명이상인소대저온감성단백매적생산균유영향.재단인소기출상설계4인소3수평적정교실험,학정B.Sub3적최가발효조건위：포도당5 g/L,단백동5 g/L,효모분3 g/L,pH=8.0,접충비례위5%,50 mL삼각병장액량위20 mL,32℃발효28 h,발효액적저온단백매활력위2 231 U/mL.
The strains of producing Low-temperature alkaline protease were breeded and the conditions of fermentation were studied.The initial strains were Bacillus subtilis,which was preserved in microbiology laboratory.Strains were breeded by UV mutagenesis,and one strain was obtained,labeling B.Sub 3.The nutrition conditions were examined and the results showed that the composition of the fermentation medium was glucose 5 g/L,peptone 5 g/L,yeast power 3 g/L,pH 8.0.The conditions of fermentation were optimized,and the results showed that the optimal fermentation condition was 5 % inoculation amount in 10 mL of medium filled in 50 mL flasks for 28 h.The production of breeding strains was considerable,and the activity of low-temperature alkaline protease reached 2 231 U/mL at this optimal fermentation condition.
