热带生物学报
熱帶生物學報
열대생물학보
JOURNAL OF SOUTH CHINA UNIVERSITY OF TROPICAL AGRICULTURE
2011年
2期
143-147
,共5页
曹嵩晓%李碧英%李娟玲%刘国民%韦江云
曹嵩曉%李碧英%李娟玲%劉國民%韋江雲
조숭효%리벽영%리연령%류국민%위강운
广藿香%丛生芽%组织培养%快繁技术%移栽
廣藿香%叢生芽%組織培養%快繁技術%移栽
엄곽향%총생아%조직배양%쾌번기술%이재
Pogostemon cablin%clustered shoots%rapid propagation via tissue culture%transplantation
以广藿香幼嫩叶片为外植体,通过愈伤组织途经诱导出大量丛生芽,然后将丛生芽分别转接到含有不同质量浓度6-BA的增殖培养基和含有不同质量浓度IBA的生根培养基上,筛选出最适增殖培养基:MS+6-BA 0.1~0.2 mg.L-1,最适生根培养基:1/2 MS+IBA 0.25~1.0 mg.L-1。广藿香组培苗株高达5~6 cm时进行炼苗移栽,移栽成活率高达95%。
以廣藿香幼嫩葉片為外植體,通過愈傷組織途經誘導齣大量叢生芽,然後將叢生芽分彆轉接到含有不同質量濃度6-BA的增殖培養基和含有不同質量濃度IBA的生根培養基上,篩選齣最適增殖培養基:MS+6-BA 0.1~0.2 mg.L-1,最適生根培養基:1/2 MS+IBA 0.25~1.0 mg.L-1。廣藿香組培苗株高達5~6 cm時進行煉苗移栽,移栽成活率高達95%。
이엄곽향유눈협편위외식체,통과유상조직도경유도출대량총생아,연후장총생아분별전접도함유불동질량농도6-BA적증식배양기화함유불동질량농도IBA적생근배양기상,사선출최괄증식배양기:MS+6-BA 0.1~0.2 mg.L-1,최괄생근배양기:1/2 MS+IBA 0.25~1.0 mg.L-1。엄곽향조배묘주고체5~6 cm시진행련묘이재,이재성활솔고체95%。
The young leaves of Pogostemon cablin were used as explants,and a large number of shoots were obtained through inducing callus.The shoots were transferred onto the multiplication media with different concentration of 6-BA,and onto the root-promoting media with different concentration of IBA.The optimum multiplication culture medium was obtained(MS +0.1 mg·L-1—0.2 mg·L-1 6-BA),and the optimum root-promoting medium was obtained(1/2 MS +0.25 mg·L-1—1.0 mg·L-1 IBA).When the height of the plantlets of Pogostemon cablin amounted to 5—6 cm,the survival percentage of plantlets Pogostemon cablin in the transplantation was 95%.