CAJ | 학술논문

利用聚丙烯酰胺凝胶电泳分析了黑龙江、吉林、辽宁和内蒙古4省(区)近10 a来通过审定的163个大豆品种的11s和7s球蛋白亚基组成、相对百分含量和11s/7S比值,并对其进行了省份问比较分析和相关性分析.结果表明:品种问大豆蛋白各亚基的相埘含量存在较大差异;163份供试材料的7s、11S球蛋白平均相对含量分别为28.95%和56.30%.变幅分别为18.40%～36.20%和47.90%～71.50%,11S/7S比值的变异幅度在1.34～3.32之间,平均值为1.97;初步筛选出α和A3缺失或稀少的特异大豆品种9份;吉林、黑龙江、辽宁和内蒙古4个地区大豆群体蛋白亚基组成及11S/7S比值存在显著差异.7S和11S组分含量问存在极显著的负相关(r=-0.2862,P<0.01).东北大豆品种贮藏蛋白7S和11S组分及其亚基相对含量存在显著变异,为大豆品质育种和豆制品加工业原料选择提供了丰富的物质基础.
이용취병희선알응효전영분석료흑룡강、길림、료녕화내몽고4성(구)근10 a래통과심정적163개대두품충적11s화7s구단백아기조성、상대백분함량화11s/7S비치,병대기진행료성빈문비교분석화상관성분석.결과표명:품충문대두단백각아기적상시함량존재교대차이;163빈공시재료적7s、11S구단백평균상대함량분별위28.95%화56.30%.변폭분별위18.40%～36.20%화47.90%～71.50%,11S/7S비치적변이폭도재1.34～3.32지간,평균치위1.97;초보사선출α화A3결실혹희소적특이대두품충9빈;길림、흑룡강、료녕화내몽고4개지구대두군체단백아기조성급11S/7S비치존재현저차이.7S화11S조분함량문존재겁현저적부상관(r=-0.2862,P<0.01).동북대두품충저장단백7S화11S조분급기아기상대함량존재현저변이,위대두품질육충화두제품가공업원료선택제공료봉부적물질기출.
The contents of 7S and US globulins and their respective sulxniits,and the 11S/7S ratios were tightly associated with the nutrition and processing properties of soybean protein. The relative percentage content of US and 7S and the 11S/7S value of 163 soybean cultivars from Northeast China were investigated by SDS-PAGE method. Results showed the content of protein subunits varied greatly among varieties, the average content and variation rage of 11S and 7S globulin were 56.30% and 28.95% ,47.90%-71.50% and 18. 40%-36. 20% .respectively; 11S/7S value ranged from 1.34 to 3. 32, with an average of 1. 97 ;nine accessions screened from 163 germplasm showed polymorphism of band type for the subunit relative contents. 11S/7S value was significantly different among the four cultivated regions; remarkably negative correlation existed between the relative contents of 7S and 1 IS globulins(r =-0.2862, P <0.01). Results suggest the subunit composition and their relative content of the storage protein 7S and US from Northeast China had remarkable variation, which would provid rich material for the soybean quality breeding.