CAJ | 학술논문

为探讨吉富罗非鱼(genetic improvement of farmed tilapia,GIFT)雌、雄群体间的遗传差异,本研究对国家级广西南宁罗非鱼良种场雌、雄吉富罗非鱼进行了遗传差异分析.研究结果表明,选取的11对SSR引物中有10对能获得稳定的目的条带;每个SSR基因座的等位基因数在2～4个之间,雌性罗非鱼的平均等位基因(N_a)2.9个,稍高于雄性的2.8个;雌、雄吉富罗非鱼平均观察杂合度(H_o)分别为0.418 3和0.415 4,多态信息含量(PIC)分别为0.404 8和0.393 2,属中度多态;雌雄个体间的遗传距离和相似性指数分别为0.090 8和0.913 2.此外,SSR基因座PRL-SO_2在雄鱼中偏离Hardy Weinberg平衡(P<0.005).上述结果表明,吉富罗非鱼雌、雄群体的SSR多态性基本相同,推测这两者基因组间的差异较小.
위탐토길부라비어(genetic improvement of farmed tilapia,GIFT)자、웅군체간적유전차이,본연구대국가급엄서남저라비어량충장자、웅길부라비어진행료유전차이분석.연구결과표명,선취적11대SSR인물중유10대능획득은정적목적조대;매개SSR기인좌적등위기인수재2～4개지간,자성라비어적평균등위기인(N_a)2.9개,초고우웅성적2.8개;자、웅길부라비어평균관찰잡합도(H_o)분별위0.418 3화0.415 4,다태신식함량(PIC)분별위0.404 8화0.393 2,속중도다태;자웅개체간적유전거리화상사성지수분별위0.090 8화0.913 2.차외,SSR기인좌PRL-SO_2재웅어중편리Hardy Weinberg평형(P<0.005).상술결과표명,길부라비어자、웅군체적SSR다태성기본상동,추측저량자기인조간적차이교소.
In this research, we have comparatively analyzed the genetic diversity of Genetic Improvement of Farmed Tilapia (GIFT) in Nanning, Guangxi tilapia farm-level seed, in order to obtain genetic informations of female and male GIFT. The results showed that there were 10 SSR primers successfully amplified with stable target bands within 11 tested SSR primers to identify the polymorphism for the tested GIFT. The number of alleles ranged was 2～4 in each SSR locus, and the average alleles in female GIFT was 2.9 slightly higher than that of 2.8 in male GIFT. Then the average of polymorphism information content (PIC), observed heterozygosity(H_o) in female and male GIFT was 0.404 8 and 0.393 2, 0.418 3 and 0.415 4 respectively, so it revealed the midrange polymorphism of fe-male and male GIFT. Genetic similarity index and genetic distance between individual of female and male GIFT was 0.090 8 and 0.913 2 respectively. We also found that SSR locus PRL-SO_2 showed significant departure from Hardy-Weinberg equilibrium after sequential bonferroni correction (P< 0.005) in male GIFT. Above-mentioned results indicated that SSR locus diversity was the same between male and female GIFT, and it was also presumed that genome variance between male and female GIFT should be the same.