解剖学报
解剖學報
해부학보
ACTA ANATOMICA SINICA
2010年
2期
237-240
,共4页
龙友国%余跃生%龙思方%江家志
龍友國%餘躍生%龍思方%江傢誌
룡우국%여약생%룡사방%강가지
短串联重复序列%遗传多态性%聚合酶链反应%贵州布依族
短串聯重複序列%遺傳多態性%聚閤酶鏈反應%貴州佈依族
단천련중복서렬%유전다태성%취합매련반응%귀주포의족
Short tandem repeat%Genetic polymorphism%PCR%Bouyei ethnic group
目的 调查贵州布依族人群CSF1PO、TPOX和TH01 3个人类短串联重复序列(STR)基因座遗传多态性.方法 用chelex-100提取法对101名布依族无关个体DNA进行提取,聚合酶链反应(PCR)复合扩增,变性聚丙烯酰胺凝胶电泳(PAGE)和银染分型,数据统计采用Modified-Powerstat统计软件. 结果 CSF1PO、TPOX和TH01基因座分别检出 8个、6个和 7个等位基因,基因型为17、15和18种,各基因型分布均符合Hardy-Weinberg平衡定律(P>0.05).CSF1PO、TPOX和TH01基因座杂合率(H)分别为0.7129、0.6238和0.7030;多态信息量(PIC)为0.6934、0.5714和0.6830; 个体识别率(DP)为0.8809、0.7929和0.8523;非父排除率(PPE)为0.5485、0.4204和0.5329. 结论 贵州布依族CSF1PO和TH01两个基因座具有较高的遗传多态性,在群体遗传学和法医学个体识别中有较高的应用价值.
目的 調查貴州佈依族人群CSF1PO、TPOX和TH01 3箇人類短串聯重複序列(STR)基因座遺傳多態性.方法 用chelex-100提取法對101名佈依族無關箇體DNA進行提取,聚閤酶鏈反應(PCR)複閤擴增,變性聚丙烯酰胺凝膠電泳(PAGE)和銀染分型,數據統計採用Modified-Powerstat統計軟件. 結果 CSF1PO、TPOX和TH01基因座分彆檢齣 8箇、6箇和 7箇等位基因,基因型為17、15和18種,各基因型分佈均符閤Hardy-Weinberg平衡定律(P>0.05).CSF1PO、TPOX和TH01基因座雜閤率(H)分彆為0.7129、0.6238和0.7030;多態信息量(PIC)為0.6934、0.5714和0.6830; 箇體識彆率(DP)為0.8809、0.7929和0.8523;非父排除率(PPE)為0.5485、0.4204和0.5329. 結論 貴州佈依族CSF1PO和TH01兩箇基因座具有較高的遺傳多態性,在群體遺傳學和法醫學箇體識彆中有較高的應用價值.
목적 조사귀주포의족인군CSF1PO、TPOX화TH01 3개인류단천련중복서렬(STR)기인좌유전다태성.방법 용chelex-100제취법대101명포의족무관개체DNA진행제취,취합매련반응(PCR)복합확증,변성취병희선알응효전영(PAGE)화은염분형,수거통계채용Modified-Powerstat통계연건. 결과 CSF1PO、TPOX화TH01기인좌분별검출 8개、6개화 7개등위기인,기인형위17、15화18충,각기인형분포균부합Hardy-Weinberg평형정률(P>0.05).CSF1PO、TPOX화TH01기인좌잡합솔(H)분별위0.7129、0.6238화0.7030;다태신식량(PIC)위0.6934、0.5714화0.6830; 개체식별솔(DP)위0.8809、0.7929화0.8523;비부배제솔(PPE)위0.5485、0.4204화0.5329. 결론 귀주포의족CSF1PO화TH01량개기인좌구유교고적유전다태성,재군체유전학화법의학개체식별중유교고적응용개치.
Objective To investigate genetic polymorphism of 3 STR loci in Bouyei ethnic group in Guizhou.Methods The DNAs of 101 healthy unrelated Bouyei individuals in Guizhou were extracted using chelex-100 method and were multiplex amplificated by PCR technique and the denatured PAGE was used, the PCR product was typied using silver stain method. The data was statistically analysed by modified-powerstat software package. Results A total of 21 alleles and 50 genotypes were observed in the 3 loci and the distributions of these genotypes were consistent with the law of Hardy-Weinberg equilibrium. The heterozygosity(H) of CSF1PO, TPOX and TH01 loci were 0.7287,0.5423 and 0.6904; the polymorphism information content(PIC) were 0.6805,0.4479 and 0.6426; the discrimination power(DP) were 0.8782,0.7361 and 0.8563 and the probabilities of paternity exclusion (PPE)were 0.6148、0.3643 and 0.5737 respectively. Conclution CSF1PO and THOX are highly genetic polymolphism, which is valuable for population genetic research and forensic individual identification. The distrbution of gene frequencies and their area of ethnic groups have parallel relation.