分析试验室
分析試驗室
분석시험실
ANALYTICAL LABORATORY
2010年
4期
83-86
,共4页
王艳芳%杨景芝%孙衍华%付蕾
王豔芳%楊景芝%孫衍華%付蕾
왕염방%양경지%손연화%부뢰
紫外分光光度法%溴氧化%维生素C%多酚
紫外分光光度法%溴氧化%維生素C%多酚
자외분광광도법%추양화%유생소C%다분
Ultraviolet spectrophotometry%Bromine%Vitamin C%Polyphenols
研究了同时测定维生素C和多酚的新方法.在体积分数38%和47.5%的乙醇、6×10~(-5) mol/L Br_2、2.5×10~(-3) mol/L KI水溶液中,维生素C和多酚类的表观摩尔吸光系数(L·mol~(-1)·cm~(-1))分别为5.42×10~4、8.22×10~5和5.46×10~4、8.60×10~5,维生素C和多酚的相对标准偏差(RSD)分别为2.5%、2.3%和2.1%、2.6%,该法用于绿豆芽、酸橙、未熟苹果、青椒中的维生素C和多酚含量测定,回收率为95.78%～100.7%.
研究瞭同時測定維生素C和多酚的新方法.在體積分數38%和47.5%的乙醇、6×10~(-5) mol/L Br_2、2.5×10~(-3) mol/L KI水溶液中,維生素C和多酚類的錶觀摩爾吸光繫數(L·mol~(-1)·cm~(-1))分彆為5.42×10~4、8.22×10~5和5.46×10~4、8.60×10~5,維生素C和多酚的相對標準偏差(RSD)分彆為2.5%、2.3%和2.1%、2.6%,該法用于綠豆芽、痠橙、未熟蘋果、青椒中的維生素C和多酚含量測定,迴收率為95.78%～100.7%.
연구료동시측정유생소C화다분적신방법.재체적분수38%화47.5%적을순、6×10~(-5) mol/L Br_2、2.5×10~(-3) mol/L KI수용액중,유생소C화다분류적표관마이흡광계수(L·mol~(-1)·cm~(-1))분별위5.42×10~4、8.22×10~5화5.46×10~4、8.60×10~5,유생소C화다분적상대표준편차(RSD)분별위2.5%、2.3%화2.1%、2.6%,해법용우록두아、산등、미숙평과、청초중적유생소C화다분함량측정,회수솔위95.78%～100.7%.
A method to determinate the concentration of VC and polyphenols without separation was studied. In 38% and 47.5% (v/v) ethanol, 6×10~(-5) mol/L Br_2, 2.5×10~(-3) mol/L KI aqueous solutions, the apparent molar absorptivities (L·mol~(-1)·cm~(-1)) of vitamin C and polyphenol are 5.42×10~4, 8.22×10~5 and 5.46×10~4, 8.60×10~5, and the relative standard deviations are 2.5%, 2.3% and 2.1%, 2.6%. This method has been successfully used to determine the contents of VC and polyphenol in mungbea sprouts, limes, unripe apples, green capsicums, and the recoveries were found to be 95.78%～100.7%, the results are satisfactory.
