CAJ | 학술논문

目的分析格林-巴利综合征(GBS)相关空肠弯曲菌细胞毒素(CDT)作用.方法 9株GBS相关空肠弯曲菌以及4株腹泻患者分离菌株的全菌蛋白分别以0.001 μg ～ 5μg/mL的浓度作用于HeLa细胞,通过观察细胞形态的改变,获得不同菌株对细胞作用的差异.结果不同菌株间产生细胞毒作用的浓度范围从0.01μg/mL到5μg/mL.结论 GBS相关菌株对HeLa细胞的细胞毒素作用与腹泻患者分离株相比没有显著差异.不同空肠弯曲菌菌株对HeLa细胞的细胞毒素作用具有菌株特异性.
목적 분석격림-파리종합정(GBS)상관공장만곡균세포독소(CDT)작용.방법 9주GBS상관공장만곡균이급4주복사환자분리균주적전균단백분별이0.001 μg ～ 5μg/mL적농도작용우HeLa세포,통과관찰세포형태적개변,획득불동균주대세포작용적차이.결과 불동균주간산생세포독작용적농도범위종0.01μg/mL도5μg/mL.결론 GBS상관균주대HeLa세포적세포독소작용여복사환자분리주상비몰유현저차이.불동공장만곡균균주대HeLa세포적세포독소작용구유균주특이성.
In order to assess the cytotoxin effect of Campylobacter jejuni (C.jejuni), the whole cell lysates from 9 strains of Guillan-Barre Syndrome (GBS)- associated C.jejuni and 4 strains of diarrhea-associated C. jejuni were co-cultivated with HeLa cells at the concentration of 0.001-5.00 μg/mL in vitro. The morphologic change of HeLa cells was observed under Olympus BX51 microscope after treatment with different concentration of bacteria lysate in the following 4 days. The morphological changes including swelling, irregularity and lysis of the affected cells over 50% was selected as the cut off for positive change and C.jejuni 81-176 and Helicobacter J99 strains were chosen as the positive and negative control. It was found that the minimum concentration to induce the positive changes in 10 strains(8 GBS associated and 2 strains from diarrhea patients)was 0.1μg/mL and 1 strain with the positive change at the minimum concentration of 1 μg/mL. There was only one GBS-associated strain causing no morphologic change on HeLa cells at the concentration of 5 μg/mL. It was evident that the cytotoxic effect of C.jejuni strains on HeLa cell was strain-specific, and there was no significant difference in the cytotoxic effect on HeLa cell between GBS-associated and diarrhea associated C.jejuni strains.