CAJ | 학술논문

采用石蜡切片法,对分布于粤西沿海的斗嫁蜮Cellana grata(Gould)的精子发生和雄性性腺的组织结构进行了研究.斗嫁蜮精子发生经历雄性性细胞的增殖、生长、成熟、变态等阶段.依据精子发生过程中生精细胞形态的变化,精子发生过程可分为精原细胞期、初级精母细胞期、次级精母细胞期、精细胞期和精子形成期等5期.雄性性腺组织主要由滤泡构成,滤泡为生殖细胞增殖、生长、成熟、变态的场所,滤泡腔中的精子经生殖输送管向外输送.显微观察与分析表明:(1)精子发生中,生长期不明显,初级精母细胞较精原细胞小;(2)生精细胞及核的大小随发育逐渐变小,核嗜碱性逐渐增强;(3)精子发生过程中的变态期长,易观察到变态过程中的不同形态的精细胞;(4)成熟精子为鞭毛型,头部短杆状,约0.5×(3～4)μm,尾部细长呈鞭毛状;(5)生长-成熟期的雄性性腺由形态较规则的(多边形)滤泡构成,滤泡界限清晰;(6)雄性滤泡壁由不同发育时期的生精细胞依次排列,较原始的生精细胞呈嗜酸性,靠近滤泡膜分布,而发育晚期的生精细胞呈嗜碱性,渐离滤泡膜分布,成熟精子群集并呈涡旋状分布于腔中;(7)成熟雄性性腺可伸入足部或肝组织中;在肝组织切片中,可观察到结构不同的4个级别的生殖输送管;8)斗嫁蜮雌雄异体,但存在雌雄同体、性转化现象.还对斗嫁蜮精子发生与成熟过程及雌雄同体的有关问题进行了探讨.
채용석사절편법,대분포우월서연해적두가역Cellana grata(Gould)적정자발생화웅성성선적조직결구진행료연구.두가역정자발생경력웅성성세포적증식、생장、성숙、변태등계단.의거정자발생과정중생정세포형태적변화,정자발생과정가분위정원세포기、초급정모세포기、차급정모세포기、정세포기화정자형성기등5기.웅성성선조직주요유려포구성,려포위생식세포증식、생장、성숙、변태적장소,려포강중적정자경생식수송관향외수송.현미관찰여분석표명:(1)정자발생중,생장기불명현,초급정모세포교정원세포소;(2)생정세포급핵적대소수발육축점변소,핵기감성축점증강;(3)정자발생과정중적변태기장,역관찰도변태과정중적불동형태적정세포;(4)성숙정자위편모형,두부단간상,약0.5×(3～4)μm,미부세장정편모상;(5)생장-성숙기적웅성성선유형태교규칙적(다변형)려포구성,려포계한청석;(6)웅성려포벽유불동발육시기적생정세포의차배렬,교원시적생정세포정기산성,고근려포막분포,이발육만기적생정세포정기감성,점리려포막분포,성숙정자군집병정와선상분포우강중;(7)성숙웅성성선가신입족부혹간조직중;재간조직절편중,가관찰도결구불동적4개급별적생식수송관;8)두가역자웅이체,단존재자웅동체、성전화현상.환대두가역정자발생여성숙과정급자웅동체적유관문제진행료탐토.
The spermatogenesis and male gonadal histology of Cellana grata (Gould) along the coast of west Guangdong were observed with historical section. The spermatogenesis of this shell goes through stages of proliferation, growth, maturation and metamorphism. According to the morphological transformation of seminiferous cell, the process of spermatogenesis could be divided into five stages, spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid and spermatozoon. The male gonad was composed of follicle and the sperm in the follicle cavity were expelled from efferent duct. The result from observing and analyzing shows that; (l)the stage of growth was not obvious during spermatogenesis while the primary spermatocyte were smaller than the spermatogonium, (2) the seminiferous cell and its nuclei transformed smaller during the spermatogenesis and the basophilic characteristic of the nuclei was gradually intensified, (3) The diversiform spermatid was easily observed during spermatogenesis duo to the longer metamorphism stage, (4) mature sperm was 0. 5×3~4 μm , with bacilliform head and flagellum tail,(5) the male gonad at growth and maturity stage was made of regular(polygon) follicle with explicit circumscription, (6)the male follicle wall was made up of seminiferous cell of different stage arranged in file,the primal seminiferous cells, which were acidophilic, was closer to the follicle velum,the termal seminiferous cells which were basophilic, were apart from the follicle velum, and the spermatozoa show swirl in the antrum, (7)the mature male gonad can embed into the tissue of foot and liver. Four structures of germ efferent duct at different levels can be observed in liver tissue section, (8)Cellana grata (Gould) was dioecism,meanwhile the phenomena of hermaphrodite and sex reversal were observed.