CAJ | 학술논문

目的研究髓样细胞触发性受体(TREM)-1在急性梗阻性化脓性胆管炎(AOSC)患者外周血单核细胞(PBMC)中表达的规律及其临床意义.方法分别收集36例AOSC患者(实验组)和40例正常人(对照组)外周血单核细胞,以半定量RT-PCR检测单核细胞中TREM-1 mRNA表达:免疫细胞化学检测单核细胞中TREM-1蛋白表达;ELISA检测患者外周血中TNF-表达水平,免疫透射比浊法测C反应蛋白.结果对照组和实验组TREM-1 mRNA半定量比值分别为0.4571+-0.053和1.007+-0.252(P<0.05).免疫细胞化学观察结果示实验组TREM-1蛋白阳性表达率与对照组之间差异显著(P<0.01).实验组TNF-和C反应蛋白水平均高于对照组(P<0.01).结论人TREM-1在急性梗阻性化脓性胆管炎发病初表达明显升高,提示TREM-1在急性梗阻性化脓性胆管炎的发生中可能起重要作用.
목적 연구수양세포촉발성수체(TREM)-1재급성경조성화농성담관염(AOSC)환자외주혈단핵세포(PBMC)중표체적규률급기림상의의.방법 분별수집36례AOSC환자(실험조)화40례정상인(대조조)외주혈단핵세포,이반정량RT-PCR검측단핵세포중TREM-1 mRNA표체:면역세포화학검측단핵세포중TREM-1단백표체;ELISA검측환자외주혈중TNF-표체수평,면역투사비탁법측C반응단백.결과 대조조화실험조TREM-1 mRNA반정량비치분별위0.4571+-0.053화1.007+-0.252(P<0.05).면역세포화학관찰결과시실험조TREM-1단백양성표체솔여대조조지간차이현저(P<0.01).실험조TNF-화C반응단백수평균고우대조조(P<0.01).결론 인TREM-1재급성경조성화농성담관염발병초표체명현승고,제시TREM-1재급성경조성화농성담관염적발생중가능기중요작용.
Objective To investigate the expression pattern of human triggering receptor expressed on myeloid cells 1 (TREM-1) mRNA in peripheral blood mononuclear cells and its clinical significance in acute obstructive suppurative cholangitis (AOSC). Methods Peripheral blood mononuclear cells were collected from 36 patients with AOSC and 40 healthy adults. TREM-1 mRNA was determined by semi-quantitative RT-PCR, and TREM-1 protein by rmmunocytochemistry. Enzyme-linked immunosorbent assay (TNF-α) was used to detect the level of tumor necrosis factor-α (TNF-α), and imrnunoturbidimetry employed to detect C reactive protein. Results The expression of TREM-1 mRNA relative to β-actin was 1.007±0.252 in patients with AOSC, significantly higher than that in the healthy adults (0.457±0.053, P<0.05). The two groups also showed significantly different TREM protein expression (P<0.01). The AOSC patients exhibited significantly higher levels of TNF-α and C reactive protein than the healthy adults (P<0.01). Conclusion The expression of human TREM-1 in peripheral blood mononuclear cells is up-regulated obviously in early stage of AOSC, probably suggesting an important role of TREM-1 in the development of AOSC.