CAJ | 학술논문

为阐明水稻β-石竹烯在调节水稻、害虫及其天敌相互关系中的作用,克隆鉴定了一个水稻β-石竹烯合成酶基因OsCAS,并对其原核表达与遗传转化进行研究.该基因的cDNA全长1731 bp,包含一个1728 bp的开放阅读框,编码一个由576个氨基酸组成的蛋白,预测分子量66.5 kDa.系统进化树分析表明,水稻OsCAS基因编码蛋白的氨基酸序列与同为单子叶植物玉米的β-石竹烯合成酶氨基酸序列同源性达99%,而与其他植物(拟南芥、青篙、黄瓜)的同源性仅51%.褐飞虱为害和茉莉酸处理能明显上调OsCAS基因的表达水平.同时原核表达了OsCAS基因,并利用农杆菌转化系统获得OsCAS基因过量表达和RNAi的水稻品系,为分析OsCAS基因的生化与生物学功能奠定了基础.
위천명수도β-석죽희재조절수도、해충급기천활상호관계중적작용,극륭감정료일개수도β-석죽희합성매기인OsCAS,병대기원핵표체여유전전화진행연구.해기인적cDNA전장1731 bp,포함일개1728 bp적개방열독광,편마일개유576개안기산조성적단백,예측분자량66.5 kDa.계통진화수분석표명,수도OsCAS기인편마단백적안기산서렬여동위단자협식물옥미적β-석죽희합성매안기산서렬동원성체99%,이여기타식물(의남개、청고、황과)적동원성부51%.갈비슬위해화말리산처리능명현상조OsCAS기인적표체수평.동시원핵표체료OsCAS기인,병이용농간균전화계통획득OsCAS기인과량표체화RNAi적수도품계,위분석OsCAS기인적생화여생물학공능전정료기출.
To elucidate the role of β-caryophyllene emitted from rice plants in mediating interactions among rice, herbivores and their natural enemies, a rice β-caryophyllene synthase gene OsCAS was cloned, expressed in E. coli and over-expressed or silenced in rice plants using an Agrobacterium-based transformation system. The gene OsCAS, a 1731 bp cDNA, contains an open reading frame of 1728 bp, which encodes a protein of 576 amino acids with a calculated molecular weight of 66.5 kDa. Phylogenetic tree analysis showed that the putative rice OsCAS had a high amino acid identity (99%) to maize (Zea mays) β-caryophyllene synthase, whereas a low amino acid identity (51%) to those in other plants including Arabodopsis (Arabidopsis thaliana), Artemisia (Artemisia annua) and cucumber (Cucumis sativus). Infestation by rice brown planthopper Nilaparvata lugens (St(a)l) or jasmonic acid treatment enhanced significantly the expression levels of OsCAS gene. The recombinant OsCAS protein was expressed in E. coli and rice lines with over-expression or RNAi silencing of OsCAS gene were obtained by Agrobacterium-mediated transformation, which laid a foundation for elucidating the biochemical and ecological functions of OsCAS.