生物化学与生物物理进展
生物化學與生物物理進展
생물화학여생물물리진전
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2008年
12期
1387-1393
,共7页
张增峰%樊晓晖%李康生%黄楷%罗殿中%冯振博%韦敏怡%管轶%陈鸿霖%张锦霞
張增峰%樊曉暉%李康生%黃楷%囉殿中%馮振博%韋敏怡%管軼%陳鴻霖%張錦霞
장증봉%번효휘%리강생%황해%라전중%풍진박%위민이%관질%진홍림%장금하
A型流感病毒%唾液酸%SAα2%6Gal受体%SAα2%3Gal受体%H5N1病毒
A型流感病毒%唾液痠%SAα2%6Gal受體%SAα2%3Gal受體%H5N1病毒
A형류감병독%타액산%SAα2%6Gal수체%SAα2%3Gal수체%H5N1병독
influenza A virus,sialic acid receptors,SAα2,6Gal linkage,SAα2,3Gal linkage,H5N1
禽类流感病毒和人类流感病毒具有很强的受体识别特异性,分别与唾液酸α-2,3Gal和α-2,6Gal受体分子结合而感染各自的宿主细胞.这种受体结合特异性是流感病毒在禽类和人类之间跨种属传递的主要障碍.应用凝集素组织化学染色技术,探讨人呼吸道各解剖学部位流感病毒唾液酸受体的分布特征.结果显示,唾液酸α-2,3Gal受体,即禽类流感受体,主要分布在下呼吸道的呼吸部即呼吸细支气管和肺泡,而在主气管、支气管和细支气管仅少量分布.相反,人类流感病毒受体,唾液酸α-2,6Gal受体在气管、支气管呈高密度分布,随着支气管分级逐渐降低分布减少,至肺泡分布最少.但比较人呼吸道发育成熟过程中,唾液酸α-2,3Gal和α-2,6Gal受体的表达,未发现明显差别.禽流感H5N1病毒体外感染人呼吸道组织试验结果表明,肺泡上皮较支气管和气管上皮易感染,与唾液酸α-2,3Gal受体分布特点相符合.结果提示,人呼吸道可被禽流感病毒感染,目前H5N1病毒极少发生人传人的特点,可能与个体间上呼吸道唾液酸α-2,3Gal受体表达差异有关.
禽類流感病毒和人類流感病毒具有很彊的受體識彆特異性,分彆與唾液痠α-2,3Gal和α-2,6Gal受體分子結閤而感染各自的宿主細胞.這種受體結閤特異性是流感病毒在禽類和人類之間跨種屬傳遞的主要障礙.應用凝集素組織化學染色技術,探討人呼吸道各解剖學部位流感病毒唾液痠受體的分佈特徵.結果顯示,唾液痠α-2,3Gal受體,即禽類流感受體,主要分佈在下呼吸道的呼吸部即呼吸細支氣管和肺泡,而在主氣管、支氣管和細支氣管僅少量分佈.相反,人類流感病毒受體,唾液痠α-2,6Gal受體在氣管、支氣管呈高密度分佈,隨著支氣管分級逐漸降低分佈減少,至肺泡分佈最少.但比較人呼吸道髮育成熟過程中,唾液痠α-2,3Gal和α-2,6Gal受體的錶達,未髮現明顯差彆.禽流感H5N1病毒體外感染人呼吸道組織試驗結果錶明,肺泡上皮較支氣管和氣管上皮易感染,與唾液痠α-2,3Gal受體分佈特點相符閤.結果提示,人呼吸道可被禽流感病毒感染,目前H5N1病毒極少髮生人傳人的特點,可能與箇體間上呼吸道唾液痠α-2,3Gal受體錶達差異有關.
금류류감병독화인류류감병독구유흔강적수체식별특이성,분별여타액산α-2,3Gal화α-2,6Gal수체분자결합이감염각자적숙주세포.저충수체결합특이성시류감병독재금류화인류지간과충속전체적주요장애.응용응집소조직화학염색기술,탐토인호흡도각해부학부위류감병독타액산수체적분포특정.결과현시,타액산α-2,3Gal수체,즉금류류감수체,주요분포재하호흡도적호흡부즉호흡세지기관화폐포,이재주기관、지기관화세지기관부소량분포.상반,인류류감병독수체,타액산α-2,6Gal수체재기관、지기관정고밀도분포,수착지기관분급축점강저분포감소,지폐포분포최소.단비교인호흡도발육성숙과정중,타액산α-2,3Gal화α-2,6Gal수체적표체,미발현명현차별.금류감H5N1병독체외감염인호흡도조직시험결과표명,폐포상피교지기관화기관상피역감염,여타액산α-2,3Gal수체분포특점상부합.결과제시,인호흡도가피금류감병독감염,목전H5N1병독겁소발생인전인적특점,가능여개체간상호흡도타액산α-2,3Gal수체표체차이유관.
SAαt2,6 and SAα2,3 linked sialic acid molecules on epithelial cell membrane served as receptors for influenza virus, which axe specifically recognized by human and avian influenza viruses, respectively. The distribution of these two species of sialic acids in human respiratory tract from different anatomical sites and different age groups was investigated. The results showed that SAα2,3Gal species was prevalent in respiratory bronchiole and lung alveolar epithelium, but was infiequent in trachea, bronchus and bronchiole. On the contrary, the SAα2,6Gal species was more common in the trachea and bronchus and to a lesser degree in the alveolar epithelium. When compared the expression levels of SAα2,6Gal and α2,3Gal in the respiratory tract among different age groups, no significant difference was found. In the ex vivo H5N1 virus infection study, alveolus epithelium were found to be more susceptible to avian influenza than trachea and bronchus epithelial cells. These results suggest that the human respiratory tract, to some extent, is permissive for avian influenza viruses. The currently-observed limited human to human transmission of H5N1 virus may be associated with the different abundance of SAα2,3Gal linkages in human upper respiratory tract among individuals.
