中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2007年
41期
8411-8413
,共3页
王新%苗宗宁%黄伟%李小钢%陈小虎
王新%苗宗寧%黃偉%李小鋼%陳小虎
왕신%묘종저%황위%리소강%진소호
牙周膜细胞%双黄补%增殖分化
牙週膜細胞%雙黃補%增殖分化
아주막세포%쌍황보%증식분화
背景:牙周组织的修复再生取决于牙周膜细胞的数量和增殖分化能力.牙周膜细胞具有多向的分化潜能,可分化成成牙骨质细胞、成骨细胞和成纤维细胞,形成牙骨质、牙槽骨和牙周膜,实现牙周组织再生.目的:观察体外培养人牙周膜细胞增殖和分化过程中传统中药双黄补水提液对其功能的影响.设计:观察性实验.单位:无锡市第三人民医院中心实验室.材料:牙周膜组织(由健康青少年患者因矫正畸形门诊就诊时自愿提供);黄连、黄岑、骨碎补(中国药品检定所提供).方法:实验于2003-07/10在无锡市第三人民医院中心实验室完成.分别将粉碎的黄连、黄岑.骨碎补和蒸馏水1:10(m:V)混合,沸水回流5 h.收集提取液,粗滤后残渣再次沸水回流3 h.合并2次提取液,旋转蒸发浓缩,所得中药水提液浓度为3 kg/L.分黄连组、黄芩组、骨碎补组、黄连加黄岑组、黄连加骨碎补组、黄岑加骨碎补组、双黄补组和对照组8组进行实验.通过体外培养人牙周膜细胞,应用双黄补提取液作为辅助因子,采用MTT比色法测定细胞增殖情况,羟脯氨酸法测定胶原蛋白占总蛋白比值.主要观察指标:牙周膜细胞增殖的吸光度值及胶原蛋白占总蛋白的比值.结果:①牙周膜细胞增殖的测定结果:除黄连组外其他各组均明显促进人牙周膜细胞增殖,与对照组比较,差异明显(P＜0.05).双黄补组细胞的吸光度值增大最为明显.随着作用时间的延长,细胞的吸光度值逐渐增大,在第5天达到最大.不同时间各组间比较,差异有显著性意义(P＜0.05).②各组牙周膜细胞胶原蛋白占总蛋白的比值:除黄连组外其他各组均明显促进比值增大,与对照组比较,差异明显(P＜0.05).双黄补组比值增大最为明显.随着作用时间的延长,比值逐渐增大,在第5天达到最大.不同时间各组间比较.差异有显著性意义(P＜0.05).结论:双黄补水提液可明显促进人牙周膜细胞增殖,明显提高胶原蛋白在总蛋白中的比值,可作为人牙周膜细胞增殖的理想的中药辅助因子.
揹景:牙週組織的脩複再生取決于牙週膜細胞的數量和增殖分化能力.牙週膜細胞具有多嚮的分化潛能,可分化成成牙骨質細胞、成骨細胞和成纖維細胞,形成牙骨質、牙槽骨和牙週膜,實現牙週組織再生.目的:觀察體外培養人牙週膜細胞增殖和分化過程中傳統中藥雙黃補水提液對其功能的影響.設計:觀察性實驗.單位:無錫市第三人民醫院中心實驗室.材料:牙週膜組織(由健康青少年患者因矯正畸形門診就診時自願提供);黃連、黃岑、骨碎補(中國藥品檢定所提供).方法:實驗于2003-07/10在無錫市第三人民醫院中心實驗室完成.分彆將粉碎的黃連、黃岑.骨碎補和蒸餾水1:10(m:V)混閤,沸水迴流5 h.收集提取液,粗濾後殘渣再次沸水迴流3 h.閤併2次提取液,鏇轉蒸髮濃縮,所得中藥水提液濃度為3 kg/L.分黃連組、黃芩組、骨碎補組、黃連加黃岑組、黃連加骨碎補組、黃岑加骨碎補組、雙黃補組和對照組8組進行實驗.通過體外培養人牙週膜細胞,應用雙黃補提取液作為輔助因子,採用MTT比色法測定細胞增殖情況,羥脯氨痠法測定膠原蛋白佔總蛋白比值.主要觀察指標:牙週膜細胞增殖的吸光度值及膠原蛋白佔總蛋白的比值.結果:①牙週膜細胞增殖的測定結果:除黃連組外其他各組均明顯促進人牙週膜細胞增殖,與對照組比較,差異明顯(P＜0.05).雙黃補組細胞的吸光度值增大最為明顯.隨著作用時間的延長,細胞的吸光度值逐漸增大,在第5天達到最大.不同時間各組間比較,差異有顯著性意義(P＜0.05).②各組牙週膜細胞膠原蛋白佔總蛋白的比值:除黃連組外其他各組均明顯促進比值增大,與對照組比較,差異明顯(P＜0.05).雙黃補組比值增大最為明顯.隨著作用時間的延長,比值逐漸增大,在第5天達到最大.不同時間各組間比較.差異有顯著性意義(P＜0.05).結論:雙黃補水提液可明顯促進人牙週膜細胞增殖,明顯提高膠原蛋白在總蛋白中的比值,可作為人牙週膜細胞增殖的理想的中藥輔助因子.
배경:아주조직적수복재생취결우아주막세포적수량화증식분화능력.아주막세포구유다향적분화잠능,가분화성성아골질세포、성골세포화성섬유세포,형성아골질、아조골화아주막,실현아주조직재생.목적:관찰체외배양인아주막세포증식화분화과정중전통중약쌍황보수제액대기공능적영향.설계:관찰성실험.단위:무석시제삼인민의원중심실험실.재료:아주막조직(유건강청소년환자인교정기형문진취진시자원제공);황련、황잠、골쇄보(중국약품검정소제공).방법:실험우2003-07/10재무석시제삼인민의원중심실험실완성.분별장분쇄적황련、황잠.골쇄보화증류수1:10(m:V)혼합,비수회류5 h.수집제취액,조려후잔사재차비수회류3 h.합병2차제취액,선전증발농축,소득중약수제액농도위3 kg/L.분황련조、황금조、골쇄보조、황련가황잠조、황련가골쇄보조、황잠가골쇄보조、쌍황보조화대조조8조진행실험.통과체외배양인아주막세포,응용쌍황보제취액작위보조인자,채용MTT비색법측정세포증식정황,간포안산법측정효원단백점총단백비치.주요관찰지표:아주막세포증식적흡광도치급효원단백점총단백적비치.결과:①아주막세포증식적측정결과:제황련조외기타각조균명현촉진인아주막세포증식,여대조조비교,차이명현(P＜0.05).쌍황보조세포적흡광도치증대최위명현.수착작용시간적연장,세포적흡광도치축점증대,재제5천체도최대.불동시간각조간비교,차이유현저성의의(P＜0.05).②각조아주막세포효원단백점총단백적비치:제황련조외기타각조균명현촉진비치증대,여대조조비교,차이명현(P＜0.05).쌍황보조비치증대최위명현.수착작용시간적연장,비치축점증대,재제5천체도최대.불동시간각조간비교.차이유현저성의의(P＜0.05).결론:쌍황보수제액가명현촉진인아주막세포증식,명현제고효원단백재총단백중적비치,가작위인아주막세포증식적이상적중약보조인자.
BACKGROUND: The repair of periodontal tissue is dependent on the number and proliferation and differentiation abilities of periodontal ligament (PDL) cells. PDL cells have the potentiality of multi-directional differentiation such as cementoblast,osteoblast and fibroblast to fonn cement, alveolar bone and periodontal ligament and finally achieve periodontal tissue regeneration.OBJECTIVE:To observe the effects of Shuanghuangbu extract on the proliferation and differentiation of PDL cells.DESIGN:Observation trail.SETTING:Central Laboratory of Wuxi Third People's Hospital. MATERIALS: Periodontal tissue was provided voluntarily by the healthy young patients with deformity correction, and golden thread,skullcap,and rhizoma drynariae by the National Institute for the Control of Pharmaceutical and Biological Products.METHODS: The experiment was conducted in the Central Laboratory of Wuxi Third People's Hospital from July to October 2003.The crushed golden thread.skullcap,and rhizoma drynariae were mixed with distilled water at ratio of 1:10 (m:V),and refluxed in boiling water for 5 hours.The extract was collected,and after colation,the residue was refluxed in boiling water for another 3 hours. Both extract was blended, rotary evaporated and condensed, finally the liquid extract of 3 kg/L was obtained.There were 8 groups in the study including golden thread group, skullcap group,rhizoma drynariae group,golden thread plus skullcap group,golden thread plus rhizoma drynariae group, skullcap plus rhizoma drynariae group, Shuanghuangbu group and control group. PDL cells were cultured/n vitro assisted with Shuanghuangbu.The proliferation of cells was detected with MTT method and the ratio of collagen content in total protein was evaluated with hydroxyproline (HP).MAIN OUTCOME MEASURES:A value of proliferated PDL cells and the proportion of collagen protein in total protein.RESULTS:①Proliferation of PDL cells:Except golden thread group,all Chinese medicine promoted the proliferation of PDL cells significantly compared with control group (P＜0.05). The A value of Shuanghuangbu group was significantly increased.A value was increased with time and reached the peak on day 5.There were significant differences among each group at different time (P＜0.05).②Ratio of collagen content in total protein:Except golden thread group,the percentage was significantly increased by other Chinese medicines compared with control group (P＜0.05), especially Shuanghuangbu. The percentage was increased with time and reached the peak on day 5. There were significant differences among each group at different time (P＜0.05). CONCLUSION:The findings suggest that as a traditional Chinese herb,Shuanghuangbu can significantly stimulate the proliferation and differentiation of PDL cells.and increase the proportion of collagen content in total protein.It may act as an ideal Chinese medicine helper factor for the regeneration of PDL cells.
