CAJ | 학술논문

　　总结了在西藏高海拔地区昼夜温差大、空气湿度低、光照极强等特殊气候条件下基质移栽脱毒马铃薯组培苗的关键技术。选择河沙与羊粪混合作为基质，严格消毒灭菌，利用 MS +0.5 mg/L B9培养基培养壮苗，合理炼苗，温室外覆盖遮光率60%以上的遮阳网降低光照，温室内搭建塑料小拱棚提高温度及湿度，温度控制在10~25℃以内，空气湿度保持在85%以上，4~5月移栽。这样，可大大提高温室内组培苗基质移栽的成活率，降低成本，提高生产效率。
　　총결료재서장고해발지구주야온차대、공기습도저、광조겁강등특수기후조건하기질이재탈독마령서조배묘적관건기술。선택하사여양분혼합작위기질，엄격소독멸균，이용 MS +0.5 mg/L B9배양기배양장묘，합리련묘，온실외복개차광솔60%이상적차양망강저광조，온실내탑건소료소공붕제고온도급습도，온도공제재10~25℃이내，공기습도보지재85%이상，4~5월이재。저양，가대대제고온실내조배묘기질이재적성활솔，강저성본，제고생산효솔。
Key techniques for transplanting virus-free in vitro plantlets were discussed under special climatic conditions, such as high altitude, large temperature difference between daytime and nighttime, low air humidity and extremely strong sunlight. The mixture of river sand and sheep manure is used as the substrate, which wil be sterilized; MS + 0.5 mg/L Daminozide (B9) culture medium is used for the culture of sound seedling and hardening-seedling is properly carried out; the greenhouse is covered by a shading net with the shading coefficient of 60% to reduce the sunlight; a smal shed is set up in the greenhouse to increase the temperature and humidity, with the temperature being control ed within 10 - 25℃ and air humidity above 85%; and transplantation is carried out from April to May. Al these could greatly improve the survival rate, reduce cost and improve the production efficiency.