CAJ | 학술논문

目的:探讨表皮生长因子(epidermal growth factor,EGF)与胰岛素对小鼠体外受精(in vitro fertilization,IVF)胚胎植入前发育的影响.方法:小鼠IVF胚胎培养在添加不同浓度的EGF和/或胰岛素-葡萄糖的CZB培养基中,于hCG注射后138h检测囊胚发育率及每个囊胚的细胞数目.结果:培养基中添加0.1ng/ml EGF显著提高了小鼠扩张囊胚的形成率,而囊胚的细胞数目并未增加.EGF与胰岛素-葡萄糖共同添加对小鼠IVF囊胚发育率及囊胚细胞数目并未有显著提高.结论:EGF通过刺激细胞分化,而非细胞增殖方式促进小鼠IVF胚胎的早期发育.而各自最佳浓度的EGF与胰岛素共同添加对小鼠早胚并未有协同促进作用.
목적:탐토표피생장인자(epidermal growth factor,EGF)여이도소대소서체외수정(in vitro fertilization,IVF)배태식입전발육적영향.방법:소서IVF배태배양재첨가불동농도적EGF화/혹이도소-포도당적CZB배양기중,우hCG주사후138h검측낭배발육솔급매개낭배적세포수목.결과:배양기중첨가0.1ng/ml EGF현저제고료소서확장낭배적형성솔,이낭배적세포수목병미증가.EGF여이도소-포도당공동첨가대소서IVF낭배발육솔급낭배세포수목병미유현저제고.결론:EGF통과자격세포분화,이비세포증식방식촉진소서IVF배태적조기발육.이각자최가농도적EGF여이도소공동첨가대소서조배병미유협동촉진작용.
Objective: To evaluate influence of epidermal growth factor (EGF) and/or insulin on the preimplantation development in vitro of mouse in vitro fertilization (IVF) embryo. Methods: Mouse IVF embryos were cultured in the CZB media supplemented with EGF at different concentrations and/ or insulin-glucose. Blastocyst formation rate and the cell number per blastocyst were examed 138h after human chorionic gonadotropin (hCG) injection. Results: Supplementation of media with EGF at the concentration of 0.1ng/ml caused a significant increasing in the rate of expanded blastocyst formation, but no difference in cell number per blastocyst. Addition of EGF and insulin-glucose in the media did not promote the preimplantation development of mouse IVF embryos.Conclusion: EGF can promote the development in vitro of mouse preimplantation embryo derived from IVF by stimulating the cell differentiation, but not proliferation. However, insulin and EGF at respectively optimal concentration have no synergistic effect on the preimplantation development of mouse IVF embryos.