生物加工过程
生物加工過程
생물가공과정
CHINESE JOURNAL OF BIOPROCESS ENGINEERING
2007年
2期
17-22
,共6页
王小艳%冯世江%文先军%李晖%李春
王小豔%馮世江%文先軍%李暉%李春
왕소염%풍세강%문선군%리휘%리춘
β-D-Glucuronidase%酶学性质%多样性%甘草酸
β-D-Glucuronidase%酶學性質%多樣性%甘草痠
β-D-Glucuronidase%매학성질%다양성%감초산
分离筛选到能代谢甘草酸并产生不同产物的3株菌株,其中Penicillium sp.Li-3转化甘草酸生成GAMG,Aspergillus sp.Li-20生成GAMG和甘草次酸,Aspergillus sp.Li-62生成甘草次酸.对这3株菌表达β-D-葡萄糖醛酸苷酶的酶学性质进行了研究,结果表明:Penicillium sp.Li-3、Aspergillus sp.Li-20和Aspergillus sp.Li-62 β-D-葡萄糖醛酸苷酶最适催化pH分别为4.2～4.6,5.8和6.2,最适催化温度为50、45和55℃.Penicillium sp.Li-3表达的β-D-葡萄糖醛酸苷酶Km为0.328 μmol/L,Vmax为0.003 5 mmol/(L·min);Aspergillus sp.Li-20 β-D-葡萄糖醛酸苷酶Km为3.61 mmol/L,Vmax为0.034 mmol/(L·min);而Aspergillus sp.Li-62 β-D-葡萄糖醛酸苷酶Km为0.43 mmol/L,Vmax为0.106 mmol/(L·min).
分離篩選到能代謝甘草痠併產生不同產物的3株菌株,其中Penicillium sp.Li-3轉化甘草痠生成GAMG,Aspergillus sp.Li-20生成GAMG和甘草次痠,Aspergillus sp.Li-62生成甘草次痠.對這3株菌錶達β-D-葡萄糖醛痠苷酶的酶學性質進行瞭研究,結果錶明:Penicillium sp.Li-3、Aspergillus sp.Li-20和Aspergillus sp.Li-62 β-D-葡萄糖醛痠苷酶最適催化pH分彆為4.2～4.6,5.8和6.2,最適催化溫度為50、45和55℃.Penicillium sp.Li-3錶達的β-D-葡萄糖醛痠苷酶Km為0.328 μmol/L,Vmax為0.003 5 mmol/(L·min);Aspergillus sp.Li-20 β-D-葡萄糖醛痠苷酶Km為3.61 mmol/L,Vmax為0.034 mmol/(L·min);而Aspergillus sp.Li-62 β-D-葡萄糖醛痠苷酶Km為0.43 mmol/L,Vmax為0.106 mmol/(L·min).
분리사선도능대사감초산병산생불동산물적3주균주,기중Penicillium sp.Li-3전화감초산생성GAMG,Aspergillus sp.Li-20생성GAMG화감초차산,Aspergillus sp.Li-62생성감초차산.대저3주균표체β-D-포도당철산감매적매학성질진행료연구,결과표명:Penicillium sp.Li-3、Aspergillus sp.Li-20화Aspergillus sp.Li-62 β-D-포도당철산감매최괄최화pH분별위4.2～4.6,5.8화6.2,최괄최화온도위50、45화55℃.Penicillium sp.Li-3표체적β-D-포도당철산감매Km위0.328 μmol/L,Vmax위0.003 5 mmol/(L·min);Aspergillus sp.Li-20 β-D-포도당철산감매Km위3.61 mmol/L,Vmax위0.034 mmol/(L·min);이Aspergillus sp.Li-62 β-D-포도당철산감매Km위0.43 mmol/L,Vmax위0.106 mmol/(L·min).
