CAJ | 학술논문

背景:由于儿童脑的发育尚未成熟,加上血脑屏障发育不完善,临床处理不当将影响其生长发育.对未成熟脑损伤性神经变性的进一步研究是十分必要的.目的:通过建立新生7 d SD大鼠顶叶皮质脑挫伤动物模型,观察大鼠同侧顶叶皮质和海马神经细胞的超微结构.设计:完全随机对照实验.单位:上海交通大学医学院神经形态实验室和细胞生物学实验室以及中科院上海生理研究所电镜室.材料:实验于2002-10/2003-06在上海第二医科大学(现称上海交通大学医学院)解剖教研室神经形态实验室、细胞生物学实验室以及中科院上海生理研究所电镜室完成.19只新生7 d SD大鼠,随机分为实验组15只、手术对照组2只和正常对照组2只. 方法:实验组用自由落体脑外伤装置建立新生7 d SD大鼠顶叶皮质脑挫伤动物模型.手术对照组除无自由落体重力锤撞击外,麻醉、头部皮肤切开等操作与实验组相同.正常对照组动物不做任何处理.经常规电镜处理,于透射电镜下观察细胞结构的变化.主要观察指标:各组大鼠同侧顶叶皮质和海马神经细胞超微结构观察.结果:19只大鼠均进入结果分析.①电镜下,实验组神经元有两种类型的形态改变.其一为神经元树突和胞体呈巨大膨胀,伴随着细胞器的改变.早期内质网池扩大,线粒体致密和浓缩.此后内质网空泡化,线粒体进行性肿、胀和空泡化,多聚核糖体从粗面内质网上解离,并散在于胞浆中,核的改变出现于胞浆改变明显之后.核染色质由簇状集聚于核膜下呈钟面排列到向中央积聚成轮廓不规则的团块.轴突基本正常.变化二为胞浆和胞核均浓缩,胞浆中有大小不等的空泡.②手术对照组和正常对照组同侧顶叶皮质和海马细胞均无异常改变.结论:脑损伤后脑细胞肿胀、胞浆和胞核均浓缩,对未成熟脑创伤性神经变性起十分重要的作用. 揹景:由于兒童腦的髮育尚未成熟,加上血腦屏障髮育不完善,臨床處理不噹將影響其生長髮育.對未成熟腦損傷性神經變性的進一步研究是十分必要的.目的:通過建立新生7 d SD大鼠頂葉皮質腦挫傷動物模型,觀察大鼠同側頂葉皮質和海馬神經細胞的超微結構.設計:完全隨機對照實驗.單位:上海交通大學醫學院神經形態實驗室和細胞生物學實驗室以及中科院上海生理研究所電鏡室.材料:實驗于2002-10/2003-06在上海第二醫科大學(現稱上海交通大學醫學院)解剖教研室神經形態實驗室、細胞生物學實驗室以及中科院上海生理研究所電鏡室完成.19隻新生7 d SD大鼠,隨機分為實驗組15隻、手術對照組2隻和正常對照組2隻. 方法:實驗組用自由落體腦外傷裝置建立新生7 d SD大鼠頂葉皮質腦挫傷動物模型.手術對照組除無自由落體重力錘撞擊外,痳醉、頭部皮膚切開等操作與實驗組相同.正常對照組動物不做任何處理.經常規電鏡處理,于透射電鏡下觀察細胞結構的變化.主要觀察指標:各組大鼠同側頂葉皮質和海馬神經細胞超微結構觀察.結果:19隻大鼠均進入結果分析.①電鏡下,實驗組神經元有兩種類型的形態改變.其一為神經元樹突和胞體呈巨大膨脹,伴隨著細胞器的改變.早期內質網池擴大,線粒體緻密和濃縮.此後內質網空泡化,線粒體進行性腫、脹和空泡化,多聚覈糖體從粗麵內質網上解離,併散在于胞漿中,覈的改變齣現于胞漿改變明顯之後.覈染色質由簇狀集聚于覈膜下呈鐘麵排列到嚮中央積聚成輪廓不規則的糰塊.軸突基本正常.變化二為胞漿和胞覈均濃縮,胞漿中有大小不等的空泡.②手術對照組和正常對照組同側頂葉皮質和海馬細胞均無異常改變.結論:腦損傷後腦細胞腫脹、胞漿和胞覈均濃縮,對未成熟腦創傷性神經變性起十分重要的作用.
배경:유우인동뇌적발육상미성숙,가상혈뇌병장발육불완선,림상처리불당장영향기생장발육.대미성숙뇌손상성신경변성적진일보연구시십분필요적.목적:통과건립신생7 d SD대서정협피질뇌좌상동물모형,관찰대서동측정협피질화해마신경세포적초미결구.설계:완전수궤대조실험.단위:상해교통대학의학원신경형태실험실화세포생물학실험실이급중과원상해생리연구소전경실.재료:실험우2002-10/2003-06재상해제이의과대학(현칭상해교통대학의학원)해부교연실신경형태실험실、세포생물학실험실이급중과원상해생리연구소전경실완성.19지신생7 d SD대서,수궤분위실험조15지、수술대조조2지화정상대조조2지. 방법:실험조용자유락체뇌외상장치건립신생7 d SD대서정협피질뇌좌상동물모형.수술대조조제무자유락체중력추당격외,마취、두부피부절개등조작여실험조상동.정상대조조동물불주임하처리.경상규전경처리,우투사전경하관찰세포결구적변화.주요관찰지표:각조대서동측정협피질화해마신경세포초미결구관찰.결과:19지대서균진입결과분석.①전경하,실험조신경원유량충류형적형태개변.기일위신경원수돌화포체정거대팽창,반수착세포기적개변.조기내질망지확대,선립체치밀화농축.차후내질망공포화,선립체진행성종、창화공포화,다취핵당체종조면내질망상해리,병산재우포장중,핵적개변출현우포장개변명현지후.핵염색질유족상집취우핵막하정종면배렬도향중앙적취성륜곽불규칙적단괴.축돌기본정상.변화이위포장화포핵균농축,포장중유대소불등적공포.②수술대조조화정상대조조동측정협피질화해마세포균무이상개변.결론:뇌손상후뇌세포종창、포장화포핵균농축,대미성숙뇌창상성신경변성기십분중요적작용.
BACKGROUND: As a result of immature brain of children and their imperfect blood brain barrier, improper clinical treatment would affect growth and development of children. It is fully important to perform further investigation on immature brain injury induced neurodegeneration.OBJECTIVE: To observe the ultramicrostructure of neurons in homolateral parietal cortex and hippocampus in newborn 7-day SD rat with contusion of parietal cortex.DESIGN: Completely randomized controlled trial.SETTING: Laboratories of Nerve Morphology and Cytobiology, Medical College of Shanghai Jiaotong University; Electron Microscope Room of Institute for Physiology, Chinese Academy of Science.MATERIALS: The experiment was performed in Teaching and Research Section of Anatomy, Laboratories of Nerve Morphology and Cytobiology ofShanghai Second Medical University (Medical College of Shanghai Jiaotong University; Electron Microscope Room of Institute for Physiology of Chinese Academy of Science from October 2002 to June 2003. A total of 19newborn 7-day SD rats were randomly divided into experimental group, operation control group and normal control group with 15, 2 and 2 in each group respectively.METHODS: In experimental group, free-fall device for brain injury was used for establishing model of contusion of parietal cortex in newborn 7-day SD rat. Anesthesia and scalp incision were conducted, without using free-fall device in operation control group. But above procedures were not carried out in normal control group. The changes of ultramicrostructure were observed under transmission electron microscope after routine treatment of electron microscopic samples.MAIN OUTCOME MEASURES: Ultramicrostructure of neurons in homolateral parietal cortex and hippocampus.RESULTS: All the 19 rats entered results analysis. ① There were two sorts of morphological changes in neurons in experimental group. One was evident swelling of dendrites and bodies of neurons, accompanied with the changes of organelles. In the early stage, expansion of endoplasmic reticulum cisterna could be observed and mitochondria became compact and concentrated. Then, vacuolization of endoplasmic reticulum, progressive swelling and vacuolization of mitochondria, dissociation of polysomes from rough endoplasmic reticulum and scattering of them in cytoplasm could be seen. Changes of nucleolus presented after significant changes of cytoplasm. Nuclear chromatin clustered together under karyotheca and arranged as clockface, which were some masses with irregular contours gathering to the center. Axons were almost normal. The other was concentration of cytoplasm and nucleolus with vacuolizations of unequal size in cytoplasm. ②There was no abnormal change in neurons in homolateral parietal cortex and hippocampus both in operation control group and normal control group.CONCLUSION: Swelling of brain cell and concentration of cytoplasm and nucleolus after brain injury play important roles in brain injury-induced neurodegeneration of immature rats.