CAJ | 학술논문

应用化学发光方法研究了4种儿茶素及其组合物对超氧阴离子自由基(O2·-)和羟自由基(OH·)的清除能力,并对其之间的清除能力进行了比较.结果表明:EGCG、ECG、EGC和EC的O2·-清除率为50%时的浓度SC50分别为4.2、4.9、5.2和6.2μmol/L,而OH·清除率为50%时的浓度SCs则分别为0.22、0.25、0.26和0.30 μmol/L.4种儿茶素的浓度相同时,其O2·-和OH·清除率的大小顺序为:EGCG＞ECG＞EGC＞EC.并应用正交试验设计方法来优化4种儿茶素的组合,结果表明:清除O2·-和OH的最佳组合是EGCG:ECG:EGC:EC为3:3:1:1.EGCG对自由基清除率影响是最大和最显著的.在4种儿茶素的组合中,当EGCG所占的比例较大时,其组合物的自由基清除率比单体的自由基清除率要大.文中最后还模拟了不同茶叶中4种儿茶素所占的比例不同时,其清除自由基的能力的大小.本研究为利用儿茶素的不同配伍寻找一种更能发挥其药用功效的复合物提供了一条新的途经.
응용화학발광방법연구료4충인다소급기조합물대초양음리자자유기(O2·-)화간자유기(OH·)적청제능력,병대기지간적청제능력진행료비교.결과표명:EGCG、ECG、EGC화EC적O2·-청제솔위50%시적농도SC50분별위4.2、4.9、5.2화6.2μmol/L,이OH·청제솔위50%시적농도SCs칙분별위0.22、0.25、0.26화0.30 μmol/L.4충인다소적농도상동시,기O2·-화OH·청제솔적대소순서위:EGCG＞ECG＞EGC＞EC.병응용정교시험설계방법래우화4충인다소적조합,결과표명:청제O2·-화OH적최가조합시EGCG:ECG:EGC:EC위3:3:1:1.EGCG대자유기청제솔영향시최대화최현저적.재4충인다소적조합중,당EGCG소점적비례교대시,기조합물적자유기청제솔비단체적자유기청제솔요대.문중최후환모의료불동다협중4충인다소소점적비례불동시,기청제자유기적능력적대소.본연구위이용인다소적불동배오심조일충경능발휘기약용공효적복합물제공료일조신적도경.
The objectives of this paper were to study the free-radical scavenging abilities of four catechins and their blended composite toward superoxide radical anion (O2· - ) and hydroxyl radical (OH·), and to make a comparison with each other using a chemiluminescence technique. The results showed that EGCG, ECG, EGC and 0.22, 0.25, 0.26 and 0.30 μmol/L respectively. The scavenging rates at the same total concentration of four catechins were as follows: EGCG ＞ ECG ＞ EGC ＞ EC. In addition, orthogonal experimental was used to obtain the optimal ratio ECG: EGC: EC 3: 3: 1: 1. EGCG was the most important factor and was of the most significance. The scavenging rate of four-catechin composite was higher than those of corresponding individual catechins while EGCG in the composite was high in proportion.