CAJ | 학술논문

本实验目的是研究猴免疫缺陷病毒(SIV)引起多形核嗜中性白细胞(PMNs)凋亡的机理.实验用PCR技术扩增gag基因,用Western blot法测定p53和bcl-2基因的表达.结果显示PMNs在被SIV感染后随着保温时间的延长存活率下降,在感染后24h可以从PMNs中扩增出gag基因.PMNs中p53基因的的表达在感染后24h增加.同时bcl-2基因的表达在对照组和SIV感染组都增加,但在SIV感染组bcl-2蛋白的表达明显低于对照组.结果揭示SⅣ能够感染PMNs,p53和bcl-2基因表达的改变可能是SⅣ感染PMNs引起细胞凋亡的机理.
본실험목적시연구후면역결함병독(SIV)인기다형핵기중성백세포(PMNs)조망적궤리.실험용PCR기술확증gag기인,용Western blot법측정p53화bcl-2기인적표체.결과현시PMNs재피SIV감염후수착보온시간적연장존활솔하강,재감염후24h가이종PMNs중확증출gag기인.PMNs중p53기인적적표체재감염후24h증가.동시bcl-2기인적표체재대조조화SIV감염조도증가,단재SIV감염조bcl-2단백적표체명현저우대조조.결과게시SⅣ능구감염PMNs,p53화bcl-2기인표체적개변가능시SⅣ감염PMNs인기세포조망적궤리.
The aim of this study is to investigate the mechanisms of apoptosis in polymorphonuclear neutrophils (PMNs) induced by simian immunodeficiency virus(SIV). In this experiment, gag gene was amplified by the polymerase chain reaction and the expression of p53 and bcl-2 gene were determined by Western blot assay. The results showed that the viability of PMNs after treated with SIV was declined as the incubation time prolonged. Gag gene could be amplified from PMNs at 24 h postinfection. The expression of p53 gene in PMNs was enhanced at 24 h postinfection. At the same time, the expression of bcl-2 gene were elevated in both of control and SIV infected group, but the production of bcl-2 protein in SIV infected group was obviously lower than control. Our data indicate that PMNs could be infected with SIV and the changed expression of p53 and bcl-2 gene might be one of the mechanisms of apoptosis occurred in PMN infected with SIV.