CAJ | 학술논문

利用PCR技术从油菜Brassica napus H165基因组DNA中分离了napinB启动子。序列分析表明，扩增片段(nap300)与文献报道的napinB启动子相应区域的同源性为97%。将其与gus连接构建种子特异性表达载体，农杆菌介导转化烟草。PCR、Southern结果显示，nap300已整合到烟草基因组DNA中，获得了转基因植株。
이용PCR기술종유채Brassica napus H165기인조DNA중분리료napinB계동자。서렬분석표명，확증편단(nap300)여문헌보도적napinB계동자상응구역적동원성위97%。장기여gus련접구건충자특이성표체재체，농간균개도전화연초。PCR、Southern결과현시，nap300이정합도연초기인조DNA중，획득료전기인식주。
A fragment of 300bp (nap300) in napinB promter region was isolated by PCR from genomic DNA of Brassica napus H165. Nap300 and the corresponding sequence of napinB promoter shared 97% homology. Expression vector harboring the promoter fused with GUS gene was constructed for functional analysis. Tobacco plants were transformed by Agrobacterium tumefaciens-mediated method. PCR and Southern results showed that nap300 had been integrated into genomic DNA of tobacco successfully. Assay of GUS activity in the seeds of transgenic tobacco will be carried out to identify the function of the promoter.